Genetic structure of a lotic population of Burkolderia (Pseudomonas) cepacia
Genetic structure of a lotic population of Burkolderia (Pseudomonas) cepacia
复制标题
洋葱伯克氏杆菌（假单胞菌）群体的遗传结构
DOI：
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发表时间：
1995
影响因子：
4.4
通讯作者：
J. McArthur
中科院分区：
文献类型：
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作者：
M. Wise;L. Shimkets;J. McArthur
采用多位点酶电泳技术对采自东南部黑水河的洋葱伯克霍尔德菌（Burkholderia（Pseudomonascepacia））的8个结构基因位点的等位变异进行了研究。总体而言，213个菌株收集在断面点沿着流连续，从沉积物沿着银行和水柱。多位点酶电泳分析显示164种不同的电泳类型，整个群体的平均遗传多样性为0.574。遗传多样性值并没有改变空间沿着流连续。从典型的判别分析，马氏距离（种群之间的遗传相似性的测量）显示在沉积物采样点的亚群之间的显着差异，这表明细菌适应异质（或斑块）微地理环境。分离株的多位点连锁不平衡分析显示，等位基因之间的关联有限，这表明频繁的重组，相对于二分裂，在这个人口。此外，从本研究的数据创建的树状图和等位基因错配分布是典型的人口特征的广泛的遗传混合。我们建议B.洋葱被添加到不断增长的非强制性克隆细菌名单中。
The genetic structure of a population of Burkholderia (Pseudomonas) cepacia isolated from a southeastern blackwater stream was investigated by using multilocus enzyme electrophoresis to examine the allelic variation in eight structural gene loci. Overall, 213 isolates were collected at transect points along the stream continuum, from both the sediments along the bank and the water column. Multilocus enzyme electrophoresis analysis revealed 164 distinct electrophoretic types, and the mean genetic diversity of the entire population was 0.574. Genetic diversity values did not vary spatially along the stream continuum. From a canonical discriminant analysis, Mahalonobis distances (measurements of genetic similarity between populations) revealed significant differences among the subpopulations at the sediment sampling points, suggesting bacterial adaptation to a heterogeneous (or patchy) microgeographical environment. Multilocus linkage disequilibrium analysis of the isolates revealed only limited association between alleles, suggesting frequent recombination, relative to binary fission, in this population. Furthermore, the dendrogram created from the data of this study and the allele mismatch distribution are typical of a population characterized by extensive genetic mixing. We suggest that B. cepacia be added to the growing list of bacteria that are not obligatorily clonal.