EFFECT OF GROWTH-FACTORS ON CELL-PROLIFERATION AND EPITHELIALIZATION IN HUMAN SKIN
EFFECT OF GROWTH-FACTORS ON CELL-PROLIFERATION AND EPITHELIALIZATION IN HUMAN SKIN
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DOI：
10.1006/jsre.1995.1160
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发表时间：
1995-08-01
影响因子：
2.2
通讯作者：
HARMON, JW
中科院分区：
文献类型：
--
作者：
BHORA, FY;DUNKIN, BJ;HARMON, JW
慢性伤口无法愈合仍然是一个主要的医学问题。最近的研究表明，生长因子在促进伤口愈合中起重要作用。我们研究了碱性成纤维细胞生长因子（FGF），胰岛素样生长因子-1（IGF-1）和表皮生长因子（EGF）的促有丝分裂作用，比较它们的效果与单独培养基（MEM）在人皮肤外植体模型。建立了一种能在体外维持人表皮组织结构10天的稳定的器官培养体系。在器官培养的第1、3和7天，使用[H-3]胸苷（[H-3]thy）摄取测量DNA合成，并表示为cpm/mg干重（平均值+/- SEM）。在培养的第1天，FGF、IGF-1和EGF各自能够刺激[H-3]thy摄取（2372 +/-335 FGF，2226 +/-193 IGF-1，4037 +/-679 EGF vs 1108 +/-70 MEM，P < 0.05）。IGF-1和EGF在培养的第3天和第7天也刺激[H-3]thy摄取。进一步采用器官培养系统观察表皮生长。在第7天观察到从外植体外周（模拟伤口边缘的上皮再生）长出的角质形成细胞最长。EGF导致上皮生长的最大刺激（440 +/- 80 μ m），其次是FGF（330 +/- 56 μ m）、IGF-1（294 +/- 48 μ m）和MEM（189 +/- 50 μ m）。因此，我们假设FGF、IGF-1和EGF是伤口愈合的重要有丝分裂原，特别是EGF能够刺激上皮形成。IGF-1和EGF可能在早期和晚期伤口环境中发挥重要作用，而FGF可能在组织修复的早期事件中最重要。(C)出版社：Academic Press
The failure of chronic wounds to heal remains a major medical problem. Recent studies have suggested an important role for growth factors in promoting wound healing. We investigated the mitogenic effect of basic fibroblast growth factor (FGF), insulin-like growth factor-1 (IGF-1), and epidermal growth factor (EGF), comparing their effects with those of media alone (MEM) in a human skin explant model. A stable organ culture system for maintaining the histologic structure of human epidermis for 10 days in vitro was developed. DNA synthesis was measured on Days 1, 3, and 7 of organ culture using [H-3]thymidine ([H-3]thy) uptake and expressed as cpm/mg dry weight (mean +/- SEM). FGF, IGF-1, and EGF were each capable of stimulating [H-3]thy uptake on Day 1 of culture (2372 +/- 335 FGF, 2226 +/- 193 IGF-1, 4037 +/- 679 EGF vs 1108 +/- 70 MEM, P < 0.05). IGF-1 and EGF also stimulated [H-3]thy uptake on Days 3 and 7 of culture. The organ culture system was further employed to observe epidermal outgrowth. Longest keratinocyte outgrowth from the explant periphery (simulating epithelial regeneration from the wound edge) was observed on Day 7. EGF resulted in maximum stimulation of epithelial outgrowth (440 +/- 80 mu m), followed by FGF (330 +/- 56 mu m), IGF-1 (294 +/- 48 mu m), and MEM (189 +/- 50 mu m). We postulate, therefore, that FGF, IGF-1, and EGF are important mitogens for wound healing and that EGF in particular is capable of stimulating epithelialization. IGF-1 and EGF may play significant roles in both the early and late wound environments, while FGF may be most important during the early events of tissue repair. (C) 1995 Academic Press, Inc.