Synergistic antibacterial effect of Bi2S3 nanospheres combined with ineffective antibiotic gentamicin against methicillin-resistant Staphylococcus aureus
Synergistic antibacterial effect of Bi2S3 nanospheres combined with ineffective antibiotic gentamicin against methicillin-resistant Staphylococcus aureus
复制标题
Bi2S3纳米球联合无效抗生素庆大霉素对耐甲氧西林金黄色葡萄球菌的协同抗菌作用
DOI：
10.1016/j.jinorgbio.2016.12.005
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发表时间：
2017-03-01
影响因子：
3.9
通讯作者：
Lu, Zhong
中科院分区：
文献类型：
--
作者：
Ma, Lulu;Wu, Jie;Lu, Zhong
本文采用简单的水热法制备了尺寸为212 nm的Bi2S3纳米球。首次研究了β -内酰胺类（头孢呋辛，CXM；头孢噻肟，CTX和哌拉西林，PIP）、喹诺酮类（环丙沙星，CIP）和氨基糖苷类（庆大霉素，GEN）对临床分离的耐甲氧西林金黄色葡萄球菌（MRSA）的选择性增强抗菌作用。当GEN与Bi2S3纳米球联合使用时，对MRSA具有显著的协同生长抑制作用，而CXM、CTX、PIP和CIP则没有。拉曼光谱和Z电位研究表明，Bi2S3可以与GEN相互作用，且两者结合表现出较小的电负性，这可能导致了细菌细胞质中GEN含量的增加。此外，Bi2S3纳米微球与GEN结合可以破坏细菌膜功能，并诱导比Bi2S3或GEN单独产生更多的杀菌活性氧。细胞毒性试验表明，Bi2S3与GEN联合使用对人正常肝细胞LO2具有较低的毒性。这项工作表明，Bi2S3纳米球可以增强无效抗生素GEN对MRSA的作用，从而增强抗生素对抗MRSA感染的能力。(C) 2016 Elsevier Inc.版权所有。
In this paper, Bi2S3 nanospheres with size of 212 nm were prepared by a simple hydrothermal process. The selectively enhanced antibacterial effects of Bi2S3 nanospheres with three classes of ineffective antibiotics, beta-lactam (cefuroxime, CXM; cefotaxime, CTX and piperacillin, PIP), quinolone (ciprofloxacin, CIP) and aminoglycoside (gentamicin, GEN) against clinical isolated methicillin-resistant Staphylococcus aureus (MRSA) were investigated for the first time. GEN shows significantly synergistic growth inhibition against MRSA when combined with Bi2S3 nanospheres, while CXM, CTX, PIP and CIP do not. Raman spectroscopy and Z potential studies reveal that Bi2S3 could interact with GEN and the combination showed small electronegativity, which probably induced the increase of GEN content in cytoplasm of bacteria. Furthermore, the combination of Bi2S3 nanospheres and GEN can destroy the bacterial membrane function and induce more bactericidal reactive oxygen generation than that of Bi2S3 or GEN alone. The cytotoxicity test indicates that the combination of Bi2S3 and GEN presented low toxicity to human normal hepatocyte LO2. This work shows that Bi2S3 nanospheres can be used to enhance the action of ineffective antibiotic GEN against MRSA, thus strengthening the antibiotic capacity for fighting MRSA infections. (C) 2016 Elsevier Inc. All rights reserved.