Collagen Synthesis in vitro by Embryonic Spinal Cord Epithelium
Collagen Synthesis in vitro by Embryonic Spinal Cord Epithelium
复制标题
胚胎脊髓上皮体外合成胶原蛋白
DOI：
10.1126/science.179.4070.295
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发表时间：
1973
期刊：
影响因子：
56.9
通讯作者：
E. Hay
中科院分区：
文献类型：
--
作者：
R. Trelstad;A. Kang;A. M. Cohen;E. Hay
将鸡胚（12 - 15期）的离体脊髓与放射性脯氨酸在体外孵育。合成的蛋白质通过与添加的载体胶原共沉淀，然后通过分子筛和离子交换色谱进行分级。部分同位素标记的蛋白质被发现是仅由α1链组成的胶原分子。
Isolated spinal cords from chick embryos (stages 12 to 15) were incubated in vitro with radioactive proline. The proteins synthesized were fractionated by coprecipitation with added carrier collagen, followed by molecularsieve and ion-exchange chromatography. A portion of the isotopically labeled proteins were found to be collagen molecules consisting only of α1 chains.