Retinoic acid signalling links left-right asymmetric patterning and bilaterally symmetric somitogenesis in the zebrafish embryo
Retinoic acid signalling links left-right asymmetric patterning and bilaterally symmetric somitogenesis in the zebrafish embryo
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DOI：
10.1038/nature03512
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发表时间：
2005-05-12
期刊：
影响因子：
64.8
通讯作者：
Belmonte, JCI
中科院分区：
文献类型：
--
作者：
Kawakami, Y;Raya, A;Belmonte, JCI
在胚胎发生过程中，由于高度协调和定型的形态发生事件，细胞在空间上形成图案。在脊椎动物胚胎中，关于偏侧性的信息通过表观遗传和遗传事件的复杂级联传递到节点，随后传递到侧板中胚层，最终导致左右不对称的身体平面。同时，近轴中胚层以两侧对称的方式沿着前后轴形成同色异谱单位或体节。在这里，我们描述了斑马鱼胚胎中的侧化信息级联，并表明阻断该级联的早期步骤（在其到达侧板中胚层之前）会导致随机的左右不对称体节发生。我们还发现了一种由视黄酸信号传导介导的机制，该机制在缓冲侧化信息流对体节形成的左右进展的影响方面至关重要，从而确保双侧对称的体节发生。
During embryogenesis, cells are spatially patterned as a result of highly coordinated and stereotyped morphogenetic events. In the vertebrate embryo, information on laterality is conveyed to the node, and subsequently to the lateral plate mesoderm, by a complex cascade of epigenetic and genetic events, eventually leading to a left - right asymmetric body plan. At the same time, the paraxial mesoderm is patterned along the anterior - posterior axis in metameric units, or somites, in a bilaterally symmetric fashion. Here we characterize a cascade of laterality information in the zebrafish embryo and show that blocking the early steps of this cascade ( before it reaches the lateral plate mesoderm) results in random left - right asymmetric somitogenesis. We also uncover a mechanism mediated by retinoic acid signalling that is crucial in buffering the influence of the flow of laterality information on the left - right progression of somite formation, and thus in ensuring bilaterally symmetric somitogenesis.