Basal Ganglia and Thalamic Input From Neurons Located Within the Ventral Tier Cell Cluster Region of the Substantia Nigra Pars Compacta in the Rat
Basal Ganglia and Thalamic Input From Neurons Located Within the Ventral Tier Cell Cluster Region of the Substantia Nigra Pars Compacta in the Rat
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DOI：
10.1002/cne.22275
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发表时间：
2010-04-15
影响因子：
2.5
通讯作者：
Prensa, Lucia
中科院分区：
文献类型：
--
作者：
Cebrian, Carolina;Prensa, Lucia
黑质致密部 (SNc) 位于最尾部的多巴胺能 (DA) 腹侧层神经元形成典型的细胞簇，深深嵌入黑质网状部 (SNr)。在这里，我们检查了位于这些 SNc 细胞簇所在的 SNr 区域的 35 个神经元的传出投射。神经元细胞体注射了生物素化的葡聚糖胺，以便在矢状面或冠状面追踪每个完整的轴突。电生理指导保证示踪剂在显示典型 SNc 放电模式的神经元中喷射。此外，双重免疫荧光和免疫组织化学标记确保示踪剂沉积物位于 DA 细胞簇内。SNc 腹侧层细胞簇区域出现三种类型的投射神经元。 I 型神经元，投射至基底神经节； II 型神经元，针对基底神经节和丘脑，以及 III 型神经元，仅投射到丘脑。纹状体是大多数 I 型和 II 型神经元的目标，神经支配到达纹状体/胼胝体下条纹和基质区室。许多黑质纹状体纤维为苍白球提供侧支，较少情况下为丘脑底核提供侧支。在丘脑水平，II型和III型神经元优先靶向网状、腹侧后外侧和腹侧内侧核。我们的结果表明，DA腹侧层细胞簇所在的SNr区域包含投射到基底神经节和/或丘脑的神经元，因此表明帕金森病中黑质神经元的神经变性可能影响各种纹状体外基底神经节结构和多个丘脑原子核。 J.Comp。 Neurol 518:1283-1300, 2010。(C) 2009 Wiley-Liss, Inc
The most caudally located dopaminergic (DA) ventral tier neurons of the substantia nigra pars compacta (SNc) form typical cell clusters that are deeply embedded in the substantia nigra pars reticulata (SNr). Here we examine the efferent projections of 35 neurons located in the SNr region where these SNc cell clusters reside The neuronal cell body was injected with biotinylated dextran amine so as to trace each complete axon in the sagittal or the coronal plane. Electrophysiological guidance guaranteed that the tracer was ejected among neurons displaying a typical SNc discharge pattern. Furthermore, double immunofluorescence and immunohistochemical labeling ensured that the tracer deposits were placed within the DA cell clusters Three types of projection neurons occurred in the SNc ventral tier cell cluster region. type I neurons, projecting to basal ganglia; type II neurons, targeting both the basal ganglia and thalamus, and type III neurons, projecting only to the thalamus The striatum was targeted by most of the type I and II neurons and the innervation reached both the striosome/subcallosal streak and matrix compartments. Many nigrostriatal fibers provided collaterals to the globus pallidus and, less frequently, to the subthalamic nucleus. At a thalamic level, type II and III neurons preferentially targeted the reticular, ventral posterolateral, and ventral medial nuclei Our results reveal that the SNr region where DA ventral tier cell clusters reside harbors neurons projecting to the basal ganglia and/or the thalamus, thus suggesting that neurodegeneration of nigral neurons in Parkinson's disease might affect various extrastriatal basal ganglia structures and multiple thalamic nuclei. J. Comp. Neurol 518:1283-1300, 2010. (C) 2009 Wiley-Liss, Inc