The RNA-binding protein HOS5 and serine/arginine-rich proteins RS40 and RS41 participate in miRNA biogenesis in Arabidopsis.
The RNA-binding protein HOS5 and serine/arginine-rich proteins RS40 and RS41 participate in miRNA biogenesis in Arabidopsis.
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DOI：
10.1093/nar/gkv751
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发表时间：
2015-09-30
影响因子：
14.9
通讯作者：
Xiong L
中科院分区：
文献类型：
--
作者：
Chen T;Cui P;Xiong L
microrna是一类由具有茎环结构的初级miRNA （pri-miRNA）转录物产生的小调控rna。SERRATE （SE）和HYPONASTIC LEAVES 1 （HYL1）可以提高植物将pri-miRNA加工成成熟miRNA的准确性。HYL1的活性受FIERY2 (FRY2)/RNA聚合酶II c端结构域磷酸酶样1 （CPL1）的调控。在这里，我们发现高渗透应激基因表达5 （HOS5）和两个富含丝氨酸/精氨酸的剪接因子RS40和RS41，先前被证明参与mrna前剪接，影响miRNA亚群的生物发生。这些蛋白质是正确的miRNA链选择和维持miRNA水平所必需的。FRY2使HOS5去磷酸化，其磷酸化状态影响其亚核定位。HOS5和RS蛋白结合无内含子和含内含子的pri-miRNAs。重要的是，所有这些剪接相关因子都直接与核剪接斑点中的HYL1和SE相互作用。我们的研究结果表明，这些剪接因子直接参与了一组miRNA的生物发生。
MicroRNAs are a class of small regulatory RNAs that are generated from primary miRNA (pri-miRNA) transcripts with a stem-loop structure. Accuracy of the processing of pri-miRNA into mature miRNA in plants can be enhanced by SERRATE (SE) and HYPONASTIC LEAVES 1 (HYL1). HYL1 activity is regulated by the FIERY2 (FRY2)/RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1). Here, we discover that HIGH OSMOTIC STRESS GENE EXPRESSION 5 (HOS5) and two serine/arginine-rich splicing factors RS40 and RS41, previously shown to be involved in pre-mRNA splicing, affect the biogenesis of a subset of miRNA. These proteins are required for correct miRNA strand selection and the maintenance of miRNA levels. FRY2 dephosphorylates HOS5 whose phosphorylation status affects its subnuclear localization. HOS5 and the RS proteins bind both intronless and intron-containing pri-miRNAs. Importantly, all of these splicing-related factors directly interact with both HYL1 and SE in nuclear splicing speckles. Our results indicate that these splicing factors are directly involved in the biogenesis of a group of miRNA.