Single-molecule analysis of human telomerase monomer
Single-molecule analysis of human telomerase monomer
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DOI：
10.1038/nchembio.82
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发表时间：
2008-05-01
影响因子：
14.8
通讯作者：
Balasubramanian, Shankar
中科院分区：
文献类型：
--
作者：
Alves, David;Li, Haitao;Balasubramanian, Shankar
Human telomerase is a ribonucleoprotein that is minimally comprised of protein (hTERT) and RNA (hTR) components. We have applied single-molecule fluorescence two-color coincidence detection to characterize complex formation between fluorophore-labeled components in solution. By systematic labeling and in vitro assembly of hTERT, hTR and telomerase's DNA substrate, we have established that catalytically functional human telomerase comprises a stable hTERT: hTR: substrate interaction in a 1:1:1 absolute stoichiometry.
