LYSOZYME ACTIVITY IN HUMAN NEUTROPHILIC GRANULOCYTES
LYSOZYME ACTIVITY IN HUMAN NEUTROPHILIC GRANULOCYTES
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DOI：
10.1111/j.1365-2141.1973.tb01687.x
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发表时间：
1973-01-01
影响因子：
6.5
通讯作者：
ANDERSEN, V
中科院分区：
文献类型：
--
作者：
HANSEN, NE;ANDERSEN, V
摘要：在从人中性粒细胞中提取溶菌酶的五种不同方法(机械破碎法、冻融法、超声法、皂苷法和正丁醇法)中，正丁醇提取法获得的每细胞溶菌酶产量最高。用该方法测定了无血液病、肾脏病和感染性疾病的对照组，以及细菌感染、严重尿毒症和骨髓增殖性疾病患者的中性粒细胞溶菌酶含量，发现正常人中性粒细胞溶菌酶含量为2.7μg/10 6个。细菌感染患者中性粒细胞内溶菌酶活性下降50%，而严重尿毒症患者体内中性粒细胞溶菌酶活性下降25%。骨髓增殖性疾病患者血清中的IL-6含量正常。内中性粒细胞溶菌酶含量与感染的严重程度成反比，由中性粒细胞白细胞增多的程度来判断。对所有患者进行检查，中性粒细胞内溶菌酶活性与中毒肉芽的程度成反比。中性溶菌酶与血肌酐水平呈负相关，提示细菌感染和尿毒症抑制了骨髓中溶菌酶的合成，但不能排除其他机制。对中性粒细胞功能的影响尚不清楚。
Summary.Among five different methods for the extraction of lysozyme from human neutrophilic granulocytes (mechanical disruption, freezing‐thawing, sonication, solubilization with saponin and withn‐butanol), extraction withn‐butanol was found to give the highest lysozyme yield per cell. With this method the lysozyme content of neutrophilic granulocytes was measured in a control group with no haematological, renal or infectious disease, and in patients with bacterial infection, severe uraemia, and myeloproliferative disorders.It was found that the lysozyme concentration in normal human adults was 2.7 μg per 106neutrophils. Intraneutrophilic lysozyme in patients with bacterial infection was reduced by 50% whereas the activity in patients with severe uraemia was reduced by 25%. The content in patients with myeloproliferative disorders was normal. The intraneutrophilic lysozyme content varied inversely with the severity of the infection as judged by the degree of neutrophil leucocytosis. Taking all patients examined, the intraneutrophilic lysozyme activity varied inversely with the degree of toxic granulation. A negative correlation was also demonstrated between neutrophilic lysozyme and the serum creatinine level.It is suggested that bacterial infection and uraemia suppress lysozyme synthesis in the bone marrow, although other mechanisms cannot be excluded. The consequences for neutrophil function are unknown.