HSV-2 glycoprotein J promotes viral protein expression and virus spread
HSV-2 glycoprotein J promotes viral protein expression and virus spread
复制标题
HSV-2糖蛋白J促进病毒蛋白表达和病毒传播
DOI：
10.1016/j.virol.2018.09.004
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Hu Qinxue
中科院分区：
文献类型：
--
作者：
Liu Yalan;Guan Xinmeng;Li Chuntian;Ni Fengfeng;Luo Sukun;Wang Jun;Zhang Di;Zhang Mudan;Hu Qinxue
HSV-2的传播主要依赖于细胞间的接触。然而，潜在的机制仍有待确定。在这里，我们证明了HSV-2 GJ，它以前没有被分配特定的功能，促进HSV-2细胞间的传播和合胞体的形成。在病毒感染的背景下，GJ基因的敲除或敲除会削弱HSV-2在上皮细胞之间或从上皮细胞到神经细胞的传播，从而导致病毒产量下降，而异位表达的GJ基因则增强病毒产量。机制上，GJ上调HSV-2蛋白的表达水平，并促进病毒蛋白的表达和异源病毒如HIV-1和JEV的复制，提示HSV-2 GJ可能作为病毒蛋白表达和病毒生产的调节因子。本研究为进一步了解GJ在HSV-2复制中的作用提供了基础。
HSV-2 spread is predominantly dependent on cell-to-cell contact. However, the underlying mechanisms remain to be determined. Here we demonstrate that HSV-2 gJ, which was previously assigned no specific function, promotes HSV-2 cell-to-cell spread and syncytia formation. In the context of viral infection, knockout or knockdown of gJ impairs HSV-2 cell-to-cell spread among epithelial cells or from epithelial cells to neuronal cells, which leads to decreased virus production, whereas ectopic expression of gJ enhances virus production. Mechanistically, gJ increases the expression levels of HSV-2 proteins, and also enhances viral protein expression and replication of heterologous viruses like HIV-1 and JEV, suggesting that HSV-2 gJ likely functions as a regulator of viral protein expression and virus production. Findings in this study provide a basis for further understanding the role of gJ in HSV-2 replication.