Protein complexes gain momentum
Protein complexes gain momentum
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DOI：
10.1016/s0959-440x(02)00400-1
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发表时间：
2002-12-01
影响因子：
6.8
通讯作者：
Robinson, CV
中科院分区：
文献类型：
--
作者：
Sobott, F;Robinson, CV
在后基因组时代，结构生物学家面临的最大挑战是破译蛋白质复合物的稳定和瞬时相互作用。为了应对这一挑战，在过去的两年中，质谱法取得了重大进展。随着新方法的出现，针对定义相互作用伙伴，化学计量和拓扑结构，以及监测实时反应，质谱是很好的位置，有助于了解动态大分子复合物。
The greatest challenge to structural biologists in the post-genomic era is to decipher both stable and transient interactions of protein complexes. In response to this challenge, significant advances in mass spectrometry have been made in the past two years. With the inception of novel approaches targeted at defining interaction partners, stoichiometry and topology, as well as monitoring real-time reactions, mass spectrometry is well placed to contribute to the understanding of dynamic macromolecular complexes.