The Production of Multiple Small Peptaibol Families by Single 14-Module Peptide Synthetases in Trichoderma/Hypocrea
The Production of Multiple Small Peptaibol Families by Single 14-Module Peptide Synthetases in Trichoderma/Hypocrea
复制标题
DOI：
10.1002/cbdv.201100212
复制
发表时间：
2012-03-01
影响因子：
2.9
通讯作者：
von Doehren, Hans
中科院分区：
文献类型：
--
作者：
Degenkolb, Thomas;Aghcheh, Razieh Karimi;von Doehren, Hans
最常见的肽生素序列是11个残基的多肽醇，广泛分布于木霉/竹红菌属。经常联系在一起的是具有部分序列同一性的14个残基的多肽。木霉主要分支的三个菌株的基因组测序计划显示，存在多达三种类型的非核糖体多肽合成酶，具有7、14或1820个氨基酸添加模块。在这里，我们提供了证据，证明在T.virens，T.reesei(Teleomomph Hypocrea Jecorina)和T.treviide中发现的14个模块的NRPS类型，基于对T.reesei各自的NRPS基因的破坏，以及对它们的氨基酸激活结构域和模块的生物信息学分析，产生11个和14个残基的多肽。这些多肽的序列可以从基因序列中预测出来，并已通过对来自菌株618的11个和14个残基的多肽家族的分析而得到证实，这些多肽被命名为下丘脑菌素A(23个序列已确定，4个新序列)和B(3个序列已确定，2个新序列)，以及最近从T.virens中建立的毛霉素A。11-和14-残基产物的分布是菌株特有的，也取决于生长条件。讨论了模块跳转的可能机制。
The most common sequences of peptaibiotics are 11-residue peptaibols found widely distributed in the genus Trichoderma/Hypocrea. Frequently associated are 14-residue peptaibols sharing partial sequence identity. Genome sequencing projects of three Trichoderma strains of the major clades reveal the presence of up to three types of nonribosomal peptide synthetases with 7, 14, or 1820 amino acid-adding modules. Here, we provide evidence that the 14-module NRPS type found in T. virens, T. reesei (teleomorph Hypocrea jecorina), and T. atroviride produces both 11- and 14-residue peptaibols based on the disruption of the respective NRPS gene of T. reesei, and bioinformatic analysis of their amino acid-activating domains and modules. The sequences of these peptides may be predicted from the gene sequences and have been confirmed by analysis of families of 11- and 14-residue peptaibols from the strain 618, termed hypojecorins A (23 sequences determined, 4 new) and B (3 sequences determined, 2 new), and the recently established trichovirins A from T. virens. The distribution of 11- and 14-residue products is strain-specific and depends on growth conditions as well. Possible mechanisms of module skipping are discussed.