Low-energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway
Low-energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway
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DOI：
10.1002/lsm.20589
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
Hsu, Shan-Hui
中科院分区：
文献类型：
--
作者：
Chen, Chung-Huang;Hung, Huey-Shan;Hsu, Shan-Hui
背景和目标：因此，本研究的目的是确定低能量激光照射（LELI）可能通过PI 3激酶/eNOS信号通路发挥其血管生成作用并诱导内皮细胞迁移和新血管形成（伤口愈合的重要和必要部分）的机制。提出了氦氖激光对内皮细胞损伤的可能分子机制。用632.5nm的He-Ne激光照射人脐静脉内皮细胞（HU-VEC），观察其对细胞增殖、一氧化氮（NO）分泌和细胞迁移的影响。0.26J/cm ~ 2辐照能促进HUVEC的迁移能力。这与辐照诱导黏着斑蛋白表达的规律相一致。此外，还促进了血管生成。LY 294002可抑制eNOS的表达，提示He-Ne激光可通过PI 3 K途径在细胞和分子水平上调eNOS的表达。研究表明，LEM增加内皮细胞增殖、迁移、NO分泌，并确定PI 3 K-/Akt途径的激活是在LELI Lasers Surg.Med.40：46-54，2008上eNOS表达升高的关键步骤。(C)2008 Wiley-Liss，Inc.
Background and Objectives: The purpose of this study, therefore, was to determine the mechanisms by which low-energy laser irradiation (LELI) may exert some of its angiogenic effects via the PI3 kinase/eNOS signaling pathway and induce endothelial cell migration and neo-vascularization, an important and necessary part of wound healing.Study Design/Materials and Methods: The possible molecular mechanism of helium-neon (He-Ne) laser irradiation on endothelial cells was proposed. He-Ne laser at 632.5 nm was used to stimulate human umbilical vein endothelial cell (HU-VEC), and its effect on cell proliferation, nitric oxide secretion, and cell migration was determined.Results: Irradiation enhanced endothelial nitric oxidase synthase (eNOS) protein expression, and irradiation of less than 0.26 J/cm(2) enhanced eNOS gene expression in HUVEC. The cell migration ability was promoted for HUVEC irradiated with 0.26 J/cm(2). This agreed with the vinculin protein expression induced by irradiation. In addition, the angiogenesis was promoted. The induced eNOS expression was inhibited by LY294002, indicating that the effect of laser on EC could be attributed to the upregulation of eNOS expression through PI3K pathway at the cellular and molecular levels as a result of the He-Ne laser.Conclusions: The study has shown that LEM increased endothelial cell proliferation, migration, NO secretion, and identified that activation of PI3K-/Akt pathway was a critical step for the elevated for eNOS expression upon LELI Lasers Surg. Med. 40:46-54, 2008. (C) 2008 Wiley-Liss, Inc.