Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor.
Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor.
复制标题
蛋白质精氨酸甲基转移酶 6 参与精子发生过程中生殖细胞的活力并受雄激素受体下调。
DOI：
10.3390/ijms161226186
复制
发表时间：
2015-12-10
影响因子：
5.6
通讯作者：
Gui Y
中科院分区：
文献类型：
--
作者：
Luo M;Li Y;Guo H;Lin S;Chen J;Ma Q;Gu Y;Jiang Z;Gui Y
雄激素和雄激素受体（AR）对精子发生和男性生育力具有重要意义。AR敲除（ARKO）小鼠显示出对雄激素和男性不育完全不敏感;然而，这种效应的确切分子机制仍不清楚。在这项研究中，我们发现与野生型（WT）小鼠相比，ARKO小鼠睾丸中Prmt 6 mRNA和蛋白的表达水平显著上调。免疫荧光染色显示PRMT 6主要定位于精原细胞和精母细胞的细胞核。此外，荧光素酶测定数据显示，AR与睾酮一起处理通过与Prmt 6启动子的雄激素响应元件（ARE）结合来抑制Prmt 6转录。此外，Prmt 6的敲低抑制生殖细胞迁移并促进凋亡。此外，这两种细胞活性不能通过睾酮处理来增强。总之，这些数据表明，PRMT 6，这是下调AR和影响细胞迁移和生殖细胞的凋亡，可能在精子发生中发挥潜在的重要作用。
Androgens and the androgen receptor (AR) are of great importance to spermatogenesis and male fertility. AR knockout (ARKO) mice display a complete insensitivity to androgens and male infertility; however, the exact molecular mechanism for this effect remains unclear. In this study, we found that the expression levels of Prmt6 mRNA and protein were significantly up-regulated in the testes of ARKO mice compared to wild type (WT) mice. PRMT6 was principally localized to the nucleus of spermatogonia and spermatocytes by immunofluorescence staining. Furthermore, luciferase assay data showed that AR together with testosterone treatment suppressed Prmt6 transcription via binding to the androgen-responsive element (ARE) of the Prmt6 promoter. Moreover, knockdown of Prmt6 suppressed germ cells migration and promoted apoptosis. In addition, both of these cellular activities could not be enhanced by testosterone treatment. Taken together, these data indicate that PRMT6, which was down-regulated by AR and influenced cell migration and apoptosis of germ cells, could play a potentially important role in spermatogenesis.