Biodegradation of Heavy Oil in Oilfield Wastewater by Bacteria Isolated from Heavy Oil Reservoir Production Water
Biodegradation of Heavy Oil in Oilfield Wastewater by Bacteria Isolated from Heavy Oil Reservoir Production Water
复制标题
稠油油藏采出水中分离细菌生物降解油田废水中的稠油
DOI：
10.1166/jbmb.2017.1676
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发表时间：
2017-08
影响因子：
0.5
通讯作者：
Yang Jie
中科院分区：
文献类型：
--
作者：
Chen Qingguo;Kang Mengmeng;Liu Zeyue;Liu Mei;Yang Jie
从稠油油藏生产水中分离出四种烃降解菌株。 HD-1、HD-2和HD-4菌株被鉴定为假单胞菌属(Pseudomonas spp.)，HD-3菌株被鉴定为芽孢杆菌属(Bacillus sp.)。还研究了这些细菌对高温和高盐度的耐受性。这些细菌在40℃至65℃的温度下可以良好生长，矿化度为9500mg·L–1至11000mg·L–1。研究了单个菌株和混合菌株的原油降解效率。 4种菌株的混合物比单独菌株具有更好的降解效果，降解效率高达62.2%。采用GC-FID和GC-MS对生物降解前后的粗稠油样品进行检测。该菌株对正烷烃和芳烃具有较强的生物降解能力，平均降解效率约为50%和35%。但混合菌株的降解效率优于单一菌株，特别是对于菲、氟等难生物降解的碳氢化合物，其降解效率仍大于50%。
Four hydrocarbon-degrading strains were separated from heavy oil reservoir production water. HD-1, HD-2, and HD-4 strains were identified as Pseudomonas spp., and HD-3 strain was identified as Bacillus sp. The tolerance of these bacteria to high temperature and high salinity was also studied. These bacteria could grow well at temperatures of 40 °C to 65 °C, with mineralization of 9500 mg·L–1 to 11000 mg·L–1. The crude oil-degrading efficiency of the individual and mixed strains was studied. The mixture of four strains had better degradation than the individual strains, and the degrading efficiency was up to 62.2%. The crude heavy oil samples were detected by GC-FID and GC-MS before and after biodegradation. The bacterial strains showed strong biodegradability to normal alkanes and aromatics, and the average degrading efficiency was approximately 50% and 35%. However, the mixed strains had better degrading efficiency than the individual strains, especially for poorly biodegradable hydrocarbons, such as phenanthrenes and fluorines, of which the degrading efficiency was still greater than 50%.