Argyrophilic dark neurons represent various states of neuronal damage in brain insults: Some come to die and others survive
Argyrophilic dark neurons represent various states of neuronal damage in brain insults: Some come to die and others survive
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DOI：
10.1016/j.neuroscience.2004.02.002
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Nishino, H
中科院分区：
文献类型：
--
作者：
Ishida, K;Shimizu, H;Nishino, H
嗜银暗神经元（dn）反映了各种脑损伤后神经元的早期组织病理状态。我们研究了dn的命运，即将死亡或恢复，以下两种类型（重度和轻度损伤）的脑损伤。Wistar大鼠单侧海马CA1区注射伊博滕酸（伊博滕酸[IA]注射）或强迫游泳（swim）。然后进行argyrophiliii (DNs)-、活化caspase-3免疫-、TUNEL-和苏木精-伊红（H-E）染色和超微结构检查。注射IA后1 ~ 3小时，典型的具有螺旋状树突的dn（躯体和树突均为嗜铁性）密集堆积在注射部位周围海马CA1锥体细胞层中。在12-24 h后，仅在体细胞和近端树突中有嗜银性的dn，而在CA1区远端树突中无嗜银性dn。然而，在这个时候，在偏远地区发现了典型的DNs。3 h时，注射部位检测到caspase-3激活，12 h后达到峰值。注射后3 ~ 7 d， CA1锥体细胞层检测到TUNEL阳性细胞。在SWIM之后，在不同区域检测到“棕色”而不是“深色”神经元，最常见的是在CA1锥体细胞层。前3天没有检测到典型的DNs。在CA3锥体细胞中检测到caspase-3的活化，但未检测到tunel阳性细胞。超微结构检查显示IA注射部位锥体细胞的树突和细胞质中弥漫性分布着聚集的银颗粒。经SWIM处理后，银颗粒主要在受累细胞的线粒体上检测到。这些数据表明，DNs提供了一种神经元损伤的测量方法：典型的深色神经元与树突细胞骨架的广泛损伤将会死亡，而非典型的棕色神经元，可能在线粒体中受到干扰，主要存活。(c) 2004 ibro。Elsevier Ltd.出版。版权所有。
Argyrophilic dark neurons (DNs) reflect the early histopathological state of neurons following various brain insults. We examined the fate of DNs, about to either die or recover, following two types (heavy and light damage) of brain insult. Wistar rats were injected ibotenic acid unilaterally into the hippocampal CA1 region (ibotenic acid [IA] injection) or were forced to swim (SWIM). Argyrophil III (DNs)-, activated caspase-3 immuno-, TUNEL- and hematoxylin-eosin (H-E)-staining and ultrastructural examinations were then performed.One to three hours after IA injection, typical DNs (argyrophilic both in somata and dendrites) with corkscrew-like dendrites were densely packed in the pyramidal cell layer of hippocampal CA1 around the injection site. After 12-24 h, DNs were argyrophilic only in the somata and proximal dendrites but absent in distal dendrites in the CA1 region. However, at this time typical DNs were found in remote areas. At 3 h, caspase-3 activation was detected at the injection site, which increased to a peak level after 12 h. Three to 7 days after injection, TUNEL positive cells were detected in the CA1 pyramidal cell layer. Immediately following SWIM, "brown" rather than "dark" neurons were detected in the various areas and most frequently in the CA1 pyramidal cell layer. No typical DNs were detected over the first 3 days. Some activation of caspase-3 was detected in a few CA3 pyramidal cells but no TUNEL-positive cells were detected.Ultrastructural examination revealed a diffuse distribution of aggregated silver particles in the dendrites and cytoplasm of pyramidal cells at the sites of IA injection. After SWIM, silver particles were detected mainly on mitochondria of affected cells.These data suggest that DNs provide a measure of neuronal damage: typically dark neurons with broad damage to the cytoskeleton of dendrites would die, while non-typical brown neurons, that may have a disturbance in mitochondria, predominantly survive. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.