Amniotic fluid volume and fetal swallowing rate in sheep.
Amniotic fluid volume and fetal swallowing rate in sheep.
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绵羊羊水量和胎儿吞咽率。
DOI：
10.1152/ajpregu.1985.249.1.r133
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Longo,LD
中科院分区：
文献类型：
--
作者：
Tomoda,S;Brace,RA;Longo,LD
为探讨羊水（AF）的动态变化及其容量调节机制，在22只单胎妊娠绵羊中，测定了注入羊膜腔后125 I标记血清白蛋白（RISA）、51 Cr标记红细胞（Cr-RBC）和103 Ru标记微球的浓度，并测定了AF容量和胎儿吞咽率。在正常情况下，需要2-3小时RISA和Cr-RBC在AF内完全混合;然而，当胎儿死亡时，仅需要3-5小时。术后第5天，17只绵羊的房颤体积平均为975 +/- 128 ml（RISA）和986 +/- 130 ml（Cr-RBC）。用RISA和Cr-RBC测定的AF体积相关性良好。相比之下，使用微球的AF体积测量产生不稳定的结果。术后第5天，17只母羊的标记消失率平均为4.9 +/- 0.7%/h（RISA）和5.5 +/-0.7%/h（Cr-RBC），计算的胎儿吞咽率分别为935 +/- 78 ml/d（RISA）和1，085 +/- 102 ml/d（Cr-RBC）。在死胎中，消失率几乎为零，这表明标签主要通过吞咽消失。绝对吞咽量和单位胎儿体重吞咽量与胎龄相关。房颤体积与胎儿体重相关。放射性标记的白蛋白或红细胞可用于同时测量羊水量和胎儿吞咽率。此外，胎儿吞咽似乎随胎龄增加。
To investigate amniotic fluid (AF) dynamics and volume regulatory mechanisms, we measured the concentration of radioiodinated (125I) serum albumin (RISA), 51Cr-labeled red blood cells (Cr-RBC), and 103Ru-labeled microspheres after injection into the amniotic cavity and determined AF volume and fetal swallowing rate in 22 singleton pregnant sheep. Under normal conditions 2-3 h were required for complete mixing of RISA and Cr-RBC within AF; however, when the fetus was dead only 3-5 h were required. AF volume of 17 sheep on the 5th postoperative day averaged 975 +/- 128 ml by RISA and 986 +/- 130 ml by Cr-RBC. AF volume determined with RISA and Cr-RBC correlated well. In contrast, AF volume measurement with microspheres produced erratic results. The disappearance rate of the labels in 17 ewes on the 5th postoperative day averaged 4.9 +/- 0.7%/h for RISA and 5.5 +/- 0.7 for Cr-RBC, and the calculated rates of fetal swallowing were 935 +/- 78 ml/day by RISA and 1,085 +/- 102 by Cr-RBC. In dead fetuses the disappearance rates were almost zero, suggesting that the labels disappear mainly by swallowing. Absolute volume swallowed and swallowed volume per fetal weight correlated with gestational age. AF volume correlated with fetal weight. Radiolabeled albumin or red blood cells may be used to simultaneously measure amniotic fluid volume and the rate of fetal swallowing. Furthermore it appears that fetal swallowing increases with gestational age.