Effect of Prolyl-hydroxyproline (Pro-Hyp), a Food-Derived Collagen Peptide in Human Blood, on Growth of Fibroblasts from Mouse Skin
Effect of Prolyl-hydroxyproline (Pro-Hyp), a Food-Derived Collagen Peptide in Human Blood, on Growth of Fibroblasts from Mouse Skin
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DOI：
10.1021/jf802785h
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发表时间：
2009-01-28
影响因子：
6.1
通讯作者：
Sato, Kenji
中科院分区：
文献类型：
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作者：
Shigemura, Yasutaka;Iwai, Koji;Sato, Kenji
我们研究了脯氨酰-羟脯氨酸（Pro-Hyp）对小鼠皮肤成纤维细胞迁移和生长的影响，脯氨酰-羟脯氨酸（Pro-Hyp）在摄取胶原蛋白肽后出现在人外周血中。将小鼠皮肤圆片在24孔塑料板上在无胎牛血清（FBS）的培养基中培养。加入Pro-Hyp（200 nmol/mL）显著增加孵育72 h后从皮肤迁移至平板的成纤维细胞数量。加入丝裂霉素C后，Pro-Hyp的这种作用被消除。收集从小鼠皮肤迁移的成纤维细胞并在胶原凝胶上培养。即使在FBS存在下，胶原凝胶上的成纤维细胞的生长也受到抑制，而在塑料板上观察到快速的成纤维细胞生长。在含10%FBS的培养基中加入Pro-Hyp（0-1000 nmol/mL）可促进成纤维细胞在胶原凝胶上的生长，并呈剂量依赖性。这些结果表明Pro-Hyp可能刺激皮肤中成纤维细胞的生长，从而增加从皮肤迁移的成纤维细胞的数量。
We examined the effect of prolyl-hydroxyproline (Pro-Hyp), which occurs in human peripheral blood after ingestion of collagen peptide, on the migration and growth of mouse skin fibroblasts. Mouse skin discs were cultured on a 24-well plastic plate in a fetal bovine serum (FBS)-free medium. Addition of Pro-Hyp (200 nmol/mL) significantly increased the number of fibroblasts migrating from the skin to the plate after incubation for 72 h. This effect of Pro-Hyp was abolished by the addition of mitomycin C. The fibroblasts that had migrated from the mouse skin were collected and cultured on collagen gel. The growth of fibroblasts on the collagen gel was suppressed even in the presence of FBS, while rapid fibroblast growth was observed on the plastic plate. Addition of Pro-Hyp (0-1000 nmol/mL) to the medium containing 10% FBS enhanced the growth of fibroblasts on the collagen gel in a dose-dependent manner. These results suggest that Pro-Hyp might stimulate the growth of fibroblasts in the skin and consequently increase the number of fibroblasts migrating from the skin.