Molecular cloning of the bark and seed lectins from the Japanese pagoda tree (Sophora japonica)
Molecular cloning of the bark and seed lectins from the Japanese pagoda tree (Sophora japonica)
复制标题
日本槐树皮和种子凝集素的分子克隆
作者：
E. V. Van Damme;A. Barre;P. Rougé;W. Peumans
摘要克隆了编码国槐树皮和种子凝集素的cDNA，并对其进行了序列分析。从树皮中分离的polyA RNA构建的cDNA文库的筛选导致三个不同的凝集素cDNA克隆的分离。第一个克隆编码GalNAc特异性树皮凝集素，最初由Hankins等人描述，而其他克隆编码Ueno等人报道的甘露糖/葡萄糖特异性凝集素的两种亚型。种子凝集素基因的分子克隆表明，槐种子只含有一个GalNAc特异性凝集素，这是高度同源，但不相同的GalNAc特异性凝集素从树皮。所有的凝集素多肽都是从ca. 1.3 kb，其编码携带信号肽的前体。在甘露糖/葡萄糖特异性树皮凝集素的情况下，该前体在两个较小的肽中进行后处理。不同克隆的推导的氨基酸序列的比对揭示了甘露糖/葡萄糖结合和GalNAc特异性凝集素之间惊人的序列相似性。此外，有一个高度的序列同源性与其他豆科植物凝集素，允许使用的坐标豌豆，山豌豆赭色和刺桐凝集素的槐凝集素的分子建模。
AbstractcDNA clones encoding the bark and seed lectins from Sophora japonica were isolated and their sequences analyzed. Screening of a cDNA library constructed from polyA RNA isolated from the bark resulted in the isolation of three different lectin cDNA clones. The first clone encodes the GalNAc-specific bark lectin which was originally described by Hankins et al. whereas the other clones encode the two isoforms of the mannose/glucose-specific lectin reported by Ueno et al.. Molecular cloning of the seed lectin genes revealed that Sophora seeds contain only a GalNAc-specific lectin which is highly homologous to though not identical with the GalNAc-specific lectin from the bark. All lectin polypeptides are translated from mRNAs of ca. 1.3 kb encoding a precursor carrying a signal peptide. In the case of the mannose/glucose-specific bark lectins this precursor is post-translationally processed in two smaller peptides. Alignment of the deduced amino acid sequences of the different clones revealed striking sequence similarities between the mannose/glucose-binding and the GalNAc-specific lectins. Furthermore, there was a high degree of sequence homology with other legume lectins which allowed molecular modelling of the Sophora lectins using the coordinates of the Pisum sativum, Lathyrus ochrus and Erythrina corallodendron lectins.
影响因子：
2.9
作者：
DESSEN, A;GUPTA, D;SACCHETTINI, JC
通讯作者：
SACCHETTINI, JC
来自豌豆 (Pisum sativum) 的重组凝集素的设计、表达和结晶。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Prasthofer,T;Phillips,SR;Suddath,FL;Engler,JA
通讯作者：
Engler,JA