INVASION OF MESENCHYME INTO 3-DIMENSIONAL COLLAGEN GELS - A REGIONAL AND TEMPORAL ANALYSIS OF INTERACTION IN EMBRYONIC HEART-TISSUE
INVASION OF MESENCHYME INTO 3-DIMENSIONAL COLLAGEN GELS - A REGIONAL AND TEMPORAL ANALYSIS OF INTERACTION IN EMBRYONIC HEART-TISSUE
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DOI：
10.1016/0012-1606(83)90010-6
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
MARKWALD, RR
中科院分区：
文献类型：
--
作者：
RUNYAN, RB;MARKWALD, RR
在正常的[鸡]心脏发育中，房室管而不是心室的内皮细胞产生间充质细胞，这些间充质细胞在发育的64-69小时左右向心肌接种（侵入）到居间的细胞外基质中。用三维胶原基质检测房室管内皮细胞在体外的接种启动，并比较和对比心室内皮细胞的反应。在原位接种开始之前，将来自分期胚胎的房室管和心室的外植体放置在胶原凝胶的表面上。在不同的孵育时间间隔，取出外植体，在凝胶表面留下由内皮细胞组成的单层。检查内皮生长物的接种细胞。结果证实了在体内观察到的区域内皮差异。胶原凝胶的侵入是由于与胚胎心脏外植体的其他组分相互作用介导的表型改变。这种体外组织相互作用的时间过程模拟了体内接种的开始。
In normal [chicken] heart development, the endothelium of the atrioventricular canal, but not the ventricle, produces mesenchymal cells which seed (invade) into the intervening extracellular matrix toward the myocardium at around 64-69 h of development. Three-dimensional collagen substrates were used to examine the initiation of seeding by atrioventricular canal endothelia in vitro and to compare and contrast the responses of the ventricular endothelia. Explants of atrioventricular canals and ventricles from staged embryos were placed on the surfaces of collagen gels prior to the onset of seeding in situ. At varied intervals of incubation, the explant was removed, leaving behind a monolayer on the surface of the gel which consisted of endothelial cells. The endothelial outgrowths were examined for seeded cells. The results confirm the regional endothelial differences seen in vivo. Invasion of the collagen gels is due to an alteration in phenotype mediated by interaction with other components of embryonic heart explant. The time course of this tissue interaction in vitro mimics the onset of seeding in vivo.