ScGST3 and multiple R2R3-MYB transcription factors function in anthocyanin accumulation in Senecio cruentus
ScGST3 and multiple R2R3-MYB transcription factors function in anthocyanin accumulation in Senecio cruentus
复制标题
ScGST3和多种R2R3-MYB转录因子在千里光花青素积累中发挥作用
DOI：
10.1016/j.plantsci.2021.111094
复制
发表时间：
2021-10-23
期刊：
影响因子：
5.2
通讯作者：
Dai, Silan
中科院分区：
文献类型：
--
作者：
Cui, Yumeng;Fan, Jiawei;Dai, Silan
花色苷是一类重要的类黄酮类色素，与花和果实的着色有关。它们在内质网的细胞质表面合成，并被运输到液泡中储存。以前的研究表明谷胱甘肽S-转移酶（GST）参与了花青素的转运。但由于植物材料的限制，大多数GST仅参与花青素或飞燕草素的转运途径。在这里，花色素苷相关的GST，ScGST 3，是从银灰的转录组中鉴定的。ScGST 3的表达模式与舌状花中花色素苷的积累高度一致。拟南芥tt 19的分子互补表明ScGST 3的过表达恢复了突变体的花青素缺乏表型。病毒诱导的ScGST 3基因沉默（VIGS）可以抑制胭脂红和蓝色瓜拉那叶片中花色素苷的积累，进一步证实了ScGST 3在花色素苷积累中的作用。体外实验表明，ScGST 3增加了矢车菊素-3-O-葡萄糖苷（C3 G）和飞燕草素-3-O-葡萄糖苷（D3 G）的水溶性。此外，我们还确定了两个花青素相关的MYB转录因子，ScMYB 3和ScMYB 6。这两个基因的表达模式也与花青素积累高度一致。ScMYB 3和ScMYB 6基因沉默后，叶片表型发生了变化，部分结构基因的表达水平受到抑制。基于双荧光素酶试验和酵母单杂交试验的结果，ScMYB 3可以激活ScGST 3的启动子。总体而言，ScGST 3的转录受ScMYB 3的调控，ScMYB 3在瓜田螺C3 G和D3 G的转运中起重要作用。
Anthocyanins are important flavonoid pigments involved in the colouring of flowers and fruits. They are syn-thesized on the cytoplasmic surface of the endoplasmic reticulum and transported into the vacuole for storage. Previous reports have suggested that glutathione S-transferase (GST) is involved in anthocyanin transport. However, due to the limitation of plant materials, most GSTs only participate in the cyanidin or delphinidin transport pathway. Here, an anthocyanin-related GST, ScGST3, was identified from the transcriptome of ciner-aria. The expression pattern of ScGST3 was highly consistent with anthocyanin accumulation in ray florets. Molecular complementation of Arabidopsis tt19 indicated that the overexpression of ScGST3 restores the anthocyanin-deficient phenotype of the mutant. Virus-induced gene silencing (VIGS) of ScGST3 in carmine and blue cineraria leaves could inhibit anthocyanin accumulation, further confirming the function of ScGST3 in anthocyanin accumulation. In vitro assays showed that ScGST3 increases the water solubility of cyanidin-3-O-glucoside (C3G) and delphinidin-3-O-glucosid (D3G). In addition, we also identified two anthocyanin-related MYB transcription factors, ScMYB3 and ScMYB6. The expression pattern of these two genes was also highly consistent with anthocyanin accumulation. Faded abaxial leaf phenotypes were observed after the silencing of ScMYB3 and ScMYB6, and the expression levels of partial structural genes were repressed. Based on the results from dual-luciferase assays and yeast one-hybrid assays, ScMYB3 can activate the promoter of ScGST3. Collec-tively, the transcription of ScGST3 is regulated by ScMYB3, which plays an important role in the transport of C3G and D3G in cineraria.