Induction of heparanase gene expression in ventricular myocardium of rats with isoproterenol-induced cardiac hypertrophy.
Induction of heparanase gene expression in ventricular myocardium of rats with isoproterenol-induced cardiac hypertrophy.
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DOI：
10.1248/bpb.28.2331
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发表时间：
2005-12
影响因子：
2
通讯作者：
K. Kizaki;M. Okada;Ryoichi Ito;K. Yoshioka;K. Hashizume;K. Mutoh;Y. Hara
中科院分区：
文献类型：
--
作者：
K. Kizaki;M. Okada;Ryoichi Ito;K. Yoshioka;K. Hashizume;K. Mutoh;Y. Hara
在长期接受异丙肾上腺素 (ISO) 诱导的大鼠心脏肥大治疗后，检测心室中乙酰肝素酶、基质金属蛋白酶 (MMP)-2 和 MMP-9 的基因表达。大鼠接受 ISO（4 mg/kg，腹膜内注射）治疗，每天两次，持续 4 天。与对照大鼠相比，心室重量和心室重量/体重比分别增加了22%和25%。组织学显示，与对照大鼠相比，ISO 治疗大鼠的心肌细胞明显肥大。 Northern印迹杂交显示，ISO处理的大鼠心室中乙酰肝素酶和MMP-2基因转录物显着增加，而MMP-9基因表达没有被诱导。因此，长期使用 ISO 治疗后，心室中乙酰肝素酶和 MMP-2 基因表达被诱导，表明它们可能在 ISO 诱导的心脏肥大的发展中发挥重要作用。
Gene expression of heparanase, matrix metalloproteinases (MMP)-2 and MMP-9 were examined in ventricles after chronic treatment with isoproterenol (ISO) induced cardiac hypertrophy in rats. Rats were treated with ISO (4 mg/kg, intraperitoneal) twice daily for 4 d. Ventricle weight of the heart and the ventricle weight/body weight ratio were increased respectively by 22% and 25% compared with control rats. Histology showed considerable cardiomyocyte hypertrophy in the ISO-treated rats in comparison to control rats. Northern blot hybridization revealed that heparanase and MMP-2 gene transcripts increased significantly in the ventricles of ISO-treated rats, whereas MMP-9 gene expression was not induced. Thus, heparanase and MMP-2 gene expressions are induced in the ventricle after chronic treatment with ISO, indicating that they might play an important role in development of ISO-induced cardiac hypertrophy.