INDUCTION OF A NEW METALLOTHIONEIN ISOFORM (MT-IV) OCCURS DURING DIFFERENTIATION OF STRATIFIED SQUAMOUS EPITHELIA
INDUCTION OF A NEW METALLOTHIONEIN ISOFORM (MT-IV) OCCURS DURING DIFFERENTIATION OF STRATIFIED SQUAMOUS EPITHELIA
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DOI：
10.1021/bi00189a029
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发表时间：
1994-06-14
期刊：
影响因子：
2.9
通讯作者：
PALMITER, RD
中科院分区：
文献类型：
--
作者：
QUAIFE, CJ;FINDLEY, SD;PALMITER, RD
在小鼠和人体内均发现了金属硫蛋白（MT）基因家族的一个新成员，位于MT- iii基因约20 kb 5'。MT-IV蛋白在这两个物种中都是高度保守的，相对于经典的MT-I和MT-II蛋白，在第5位有一个谷氨酸插入。小鼠MT-IV mRNA似乎只在与口腔上皮、食道、上胃、尾巴、脚垫和新生儿皮肤相关的分层鳞状上皮中表达。来源于舌上皮的MT含有锌和铜。在大鼠缺锌期间，这些上皮细胞中有许多会发生异角化症。原位杂交显示，在角化上皮的分化棘层中有强烈的MT-IV mRNA标记，而MT-I主要表达在基底层和增殖层；因此，在这些上皮细胞的分化过程中，MT异构体的合成有一个开关。我们认为MT-IV在层状上皮分化过程中调节锌代谢中起特殊作用。
A new member of the metallothionein (MT) gene family was discovered that lies about 20 kb 5' of the MT-III gene in both mouse and human. The MT-IV proteins are highly conserved in both species and have a glutamate insertion at position 5 relative to the classical MT-I and MT-II proteins. Murine MT-IV mRNA appears to be expressed exclusively in stratified squamous epithelia associated with oral epithelia, esophagus, upper stomach, tail, footpads, and neonatal skin. The MT derived from tongue epithelium contains both zinc and copper. Many of these epithelia develop parakaratosis during zinc deficiency in the rat. In situ hybridization reveals intense labeling of MT-IV mRNA in the differentiating spinous layer of cornified epithelia, whereas MT-I is expressed predominantly in the basal, proliferative layer; thus, there is a switch in MT isoform synthesis during differentiation of these epithelia. We suggest that MT-IV plays a special role in regulating zinc metabolism during the differentiation of stratified epithelia.