Electrical Evaluation of DNA Stretched and Immobilized Between Triangular-Shaped Electrodes
Electrical Evaluation of DNA Stretched and Immobilized Between Triangular-Shaped Electrodes
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DOI：
10.1007/s11664-018-06899-5
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发表时间：
2019-03-01
影响因子：
2.1
通讯作者：
Saito, Yoji
中科院分区：
文献类型：
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作者：
Himuro, Takahiro;Tsukamoto, Shota;Saito, Yoji
作为迈向生物传感器应用的一步，我们通过电流-电压特性和复阻抗图表征了 lambda DNA 分子的电特性。使用微通道（深度，50μm；宽度，500μm；长度，10mm）将λDNA分子引入微流体装置中，并通过施加1MHz和20Vp-p的交流电压，静电拉伸并固定在两个三角形微光刻铝电极之间的14μm间隙中。对齐的 lambda DNA 显示出非线性电流-电压特性。根据 lambda DNA 分子的复阻抗图，获得了两个电阻电容并联电路串联的等效电路。最后，我们证明了电极之间 lambda DNA 的电特性随固定 lambda DNA 分子数量的变化而变化。
As a step toward applications for biosensors, we characterized the electrical properties of lambda DNA molecules via their current-voltage characteristics and complex impedance plots. lambda DNA molecules were introduced to a microfluidic device using a microchannel (depth, 50 mu m; width, 500 mu m; length, 10 mm) and electrostatically stretched and immobilized in the 14-mu m gap between two triangular-shaped microlithographed aluminum electrodes by applying an alternating voltage of 1 MHz and 20 Vp-p. The aligned lambda DNA showed nonlinear current-voltage characteristics. From the complex impedance plots of the lambda DNA molecules, an equivalent circuit was obtained as a series connection of two resistance-capacitance parallel circuits. Finally, we demonstrated that the electrical characteristics of the lambda DNA between the electrodes varied with the number of immobilized lambda DNA molecules.