Dynamic changes of intracellular zinc ion level during maturation, fertilization, activation, and development in mouse oocytes
Dynamic changes of intracellular zinc ion level during maturation, fertilization, activation, and development in mouse oocytes
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DOI：
10.1111/asj.13759
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发表时间：
2022-01-01
影响因子：
2
通讯作者：
Kashiwazaki，Naomi
中科院分区：
文献类型：
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作者：
Kageyama，Atsuko;Suyama，Ayumi;Kashiwazaki，Naomi
虽然众所周知，钙振荡所需的受精在所有哺乳动物物种的研究日期，最近的研究还表明，在一系列协调的事件，称为“锌火花”，在哺乳动物受精锌喷射到细胞外环境。这些结果使我们假设锌离子依赖性信号对卵母细胞成熟，受精（激活）和进一步的胚胎发育很重要。在这项研究中，我们评估了小鼠卵母细胞成熟，受精，激活和胚胎发育过程中细胞内锌离子的数量和定位。我们的研究结果表明，丰富的锌离子存在于未成熟和成熟的卵母细胞。体外受精后，锌离子的量显着下降，在原核（PN）阶段。人工活化放线菌酮，氯化锶，和TPEN也减少了量的锌离子在PN阶段。另一方面，来自精子注射的PN胚胎仍然显示出高水平的锌离子。然而，无论激活方法如何，在囊胚处锌离子的量迅速增加。我们发现，在小鼠卵母细胞的成熟、受精、激活和发育过程中，锌离子的含量发生了显著变化。
Although it is well known that calcium oscillations are required for fertilization in all mammalian species studied to date, recent studies also showed the ejection of zinc into the extracellular milieu in a series of coordinated events, called “zinc spark,” during mammalian fertilization. These results led us to the hypothesis that a zinc ion‐dependent signal is important for oocyte maturation, fertilization (activation), and further embryonic development. In this study, we evaluated the amounts and localization of intracellular zinc ions during maturation, fertilization, activation, and embryonic development in mouse oocytes. Our results show that abundant zinc ions are present in both immature and mature oocytes. After in vitro fertilization, the amounts of zinc ions were dramatically decreased at the pronuclear (PN) stage. Artificial activation by cycloheximide, SrCl2, and TPEN also reduced the amounts of zinc ions in the PN stage. On the other hand, PN embryos derived from sperm injection still showed high level of zinc ions. However, the amounts of zinc ions rapidly increased at the blastocysts regardless of activation method. We showed here that the amounts of zinc ions dramatically changed during maturation, fertilization, activation, and development in mouse oocytes.