ODORANT AND GUANINE NUCLEOTIDE-STIMULATED PHOSPHOINOSITIDE TURNOVER IN OLFACTORY CILIA
ODORANT AND GUANINE NUCLEOTIDE-STIMULATED PHOSPHOINOSITIDE TURNOVER IN OLFACTORY CILIA
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DOI：
10.1016/0006-291x(86)91172-1
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发表时间：
1986-05-29
影响因子：
3.1
通讯作者：
BRUCH, RC
中科院分区：
文献类型：
--
作者：
HUQUE, T;BRUCH, RC
从通道鲶鱼（Ictalurus punctatus）分离的嗅纤毛具有磷脂酰肌醇-4,5-二磷酸磷酸二酯酶（e.c 3.1.4.11）活性。磷酸二酯酶的活性在鲶鱼的气味，即氨基酸l -丙氨酸的存在下被刺激。GTP及其不可水解类似物的存在也刺激了酶的活性。鸟嘌呤核苷酸对磷酸二酯酶的激活，以及在离体纤毛中发现的鸟嘌呤核苷酸结合蛋白，表明鸟嘌呤核苷酸结合蛋白可能参与了刺激嗅觉受体神经元磷酸肌苷转换的过程。
Isolated olfactory cilia from the channel catfish (Ictalurus punctatus) exhibited phosphatidylinositol-4,5-bisphosphate phosphodiesterase (E.C.3.1.4.11) activity. The phosphodiesterase activity was stimulated in the presence of an odorant for the catfish, namely the amino acid L-alanine. The enzyme activity was also stimulated in the presence of GTP and its nonhydrolyzable analogues. The activation of the phosphodiesterase by guanine nucleotides, in combination with the identification of guanine nucleotide-binding protein(s) in the isolated cilia, indicate the probable participation of a guanine nucleotide-binding protein in stimulation of phosphoinositide turnover in the olfactory receptor neuron.