Effects of FSH and IGF-I on immature rat Sertoli cells: inhibin α- and β-subunit mRNA levels and inhibin secretion
Effects of FSH and IGF-I on immature rat Sertoli cells: inhibin α- and β-subunit mRNA levels and inhibin secretion
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FSH 和 IGF-I 对未成熟大鼠支持细胞的影响：抑制素 α 和 β 亚基 mRNA 水平和抑制剂分泌
DOI：
10.1016/0303-7207(88)90096-2
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发表时间：
1988
影响因子：
4.1
通讯作者：
J. Grootegoed
中科院分区：
文献类型：
--
作者：
A. Toebosch;D. Robertson;J. Trapman;P. Klaassen;R. Paus;F. Jong;J. Grootegoed
本实验观察了促卵泡激素（FSH）和胰岛素样生长因子-I（IGF-I）对培养的21 ~ 23日龄大鼠睾丸支持细胞分泌卵泡刺激素（FSH）的影响。测定了重组质粒pET 10 - 1 α和β亚基mRNA的表达、pET 10 - 1的免疫反应性和体外生物活性。用牛胰蛋白酶α亚基的cDNA探针与1.f胰蛋白酶（kb）mRNA进行特异性杂交。加入FSH培养24 h后，该mRNA的表达量呈剂量依赖性增加。IGF-I对杂交强度无影响。以人β-B1-bin β B亚基cDNA为探针，检测到3.5和4.2kbmRNA。FSH和IGF-I对杂交信号无影响。未观察到与对应于β-葡萄糖苷酶亚基的cDNA探针的杂交。通过免疫测定法检测细胞和培养基中的抑制素活性，并通过体外生物测定法检测培养基中的抑制素活性。FSH刺激免疫反应性和体外生物活性，而IGF-I没有任何影响。目前FSH对培养的Sertoli细胞中的Escherichia bin α亚基mRNA表达的影响表明FSH对Escherichia bin产生的调节包括转录水平的影响。然而，这并不排除额外的翻译和翻译后效应。
Effects of follicle-stimulating hormone (FSH) and insulin-like growth factor-I (IGF-I) on inhibin production by cultured Sertoli cells from 21- to 23-day-old rats were studied. The expression of inhibin α-and β-subunit mRNAs, and inhibin immunoreactivity and in vitro bioactivity were estimated. Using a cDNA probe corresponding to the α-subunit of bovine inhibin, specific hybridization with a 1.f kilobase (kb)mRNA species was observed. Addition of FSH to the cultured Sertoli cells for 24 h markedly increased the level of this mRNA in a dose-dependent way. IGF-I had no effect on the intensity of the hybridization. Using a cDNA probe corresponding to the βB-subunit of human inhibin 3.5 and 4.2 kb mRNA species were detected. FSH and IGF-I had no effect on the hybridization signal. No hybridization was observed with a cDNA probe corresponding to the βAbovine inhibin subunit. Inhibin activity was detected in cells and medium by immunoassay, and in the medium by in vitro bioassay. FSH stimulated both immunoreactivity and in vitro bioactivity, whereas IGF-I had no effect at all. The present effect of FSH on inhibin α-subunit mRNA expression in cultured Sertoli cells indicates that regulation of inhibin production by FSH includes an effect at the transcriptional level. However, this does not exclude additional translational and posttranslational effects.