Development and characterization of rhVEGF-loaded poly(HEMA-MOEP) coatings electrosynthesized on titanium to enhance bone mineralization and angiogenesis
Development and characterization of rhVEGF-loaded poly(HEMA-MOEP) coatings electrosynthesized on titanium to enhance bone mineralization and angiogenesis
复制标题
DOI：
10.1016/j.actbio.2009.07.008
复制
发表时间：
2010-01-01
影响因子：
9.7
通讯作者：
Mattioli-Belmonte, Monica
中科院分区：
文献类型：
--
作者：
De Giglio, Elvira;Cometa, Stefania;Mattioli-Belmonte, Monica
同时具有促进矿化和血管生成作用的涂层可以显著改善钛种植体的骨整合。在本研究中，首次在钛衬底上电合成了聚甲基丙烯酸羟乙酯-2-甲基丙烯酰氧乙基磷酸酯(P(HEMA-MOEP))共聚水凝胶涂层，以增强体液中的钙化并包裹和释放生长因子，并与空白对照的聚甲基丙烯酸羟乙酯(PHEMA)进行了比较。带有负电荷基团的聚合物，如P(HEMA-MOEP)，有助于增强种植体的钙化。用X射线光电子能谱和原子力显微镜对电合成涂层进行了表征。采用扫描电子显微镜、四甲基偶氮唑盐比色法、四甲基偶氮唑盐比色法和骨钙素信使核糖核酸检测等方法研究了MG-63人成骨样细胞在涂层上的行为。通过将植入物浸泡在模拟体液中，研究了带负电荷的磷酸盐基团促进植入物上羟基磷灰石样磷酸钙沉积的能力。两种涂层的生物反应相似，而钙磷颗粒仅在经碱性溶液处理的P(HEMA-MOEP)表面检测到。P(HEMA-MOEP)水凝胶包埋和释放重组人血管内皮生长因子的能力测试表明，P(HEMA-MOEP)涂层钛假体可能是一种适合骨修复应用的多功能材料。(C)2009年Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Osteointegration of titanium implants could be significantly improved by coatings capable of promoting both mineralization and angiogenesis. In the present study, a copolymeric hydrogel coating, poly-2-hydroxyethyl methacrylate-2-methacryloyloxyethyl phosphate (P(HEMA-MOEP)), devised to enhance calcification in body fluids and to entrap and release growth factors, was electrosynthesized for the first time on titanium substrates and compared to poly-2-hydroxyethyl methacrylate (PHEMA), used as a blank reference. Polymers exhibiting negatively charged groups, such as P(HEMA-MOEP), help to enhance implant calcification. The electrosynthesized coatings were characterized by X-ray photoelectron spectroscopy and atomic force microscopy. MG-63 human osteoblast-like cell behaviour on the coated specimens was investigated by scanning electron microscopy, MTT viability test and osteocalcin mRNA detection. The ability of negatively charged phosphate groups to promote hydroxyapatite-like calcium phosphate deposition on the implants was explored by immersing them in simulated body fluid. Similar biological responses were observed in both coated specimens, while calcium-phosphorus globules were detected only on P(HEMA-MOEP) surfaces pretreated with alkaline solution. Testing of the ability of P(HEMA-MOEP) hydrogels to entrap and release human recombinant vascular endothelial growth factor, to tackle the problem of insufficient oxygen and nutrient delivery, suggested that P(HEMA-MOEP)-coated titanium prostheses could represent a multifunctional material suitable for bone restoration applications. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.