Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and China
Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and China
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DOI：
10.1016/s0378-8741(00)00252-x
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发表时间：
2000-09-01
影响因子：
5.4
通讯作者：
Kadota, S
中科院分区：
文献类型：
--
作者：
Banskota, AH;Tezuka, Y;Kadota, S
蜂胶是一种树脂状的蜂箱产品，由蜜蜂从各种植物中收集而成。蜂胶的成分取决于采集的时间、植被和面积。因此，在食品和饮料中使用蜂胶之前，对其进行质量评价是很重要的。为此，研究了来自巴西、秘鲁、荷兰和中国的9种不同蜂胶的MeOH和水提取物的三种不同的生物活性，即1,1-二苯基-2-苦味酰肼（DPPH）自由基清除活性、细胞毒性和肝脏保护活性。结果表明，6种巴西蜂胶和1种中国蜂胶水提物对DPPH自由基的清除能力强于MeOH提取物，而荷兰和秘鲁蜂胶MeOH提取物对DPPH自由基的清除能力强于MeOH提取物。各蜂胶MeOH提取物对小鼠结肠26-L5癌和人HT-1080纤维肉瘤细胞的毒性均强于水提取物。巴西蜂胶对d -半乳糖胺(D-GalN)/肿瘤坏死因子- α (tnf - α)诱导的原代培养小鼠肝细胞死亡的保肝活性结果符合巴西养蜂人设定的等级。(C) 2000爱思唯尔科学爱尔兰有限公司版权所有。
Propolis is a resinous hive product collected by honeybees from various plant sources. The composition of the propolis depends upon the time, vegetation and the area of collection. Thus, quality evaluation of the propolis is important, before use in food and beverages. For this propose three different biological activities were carried out, i.e. 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, cytotoxicity and hepatoprotective activity, of MeOH and water extracts of nine different propolis from Brazil, Peru, the Netherlands and China. The results showed that water extracts of six Brazilian and a Chinese propolis possessed stronger DPPH free radical scavenging activity than the corresponding MeOH extract, whereas in the case of Netherlands and Peruvian propolis MeOH extract exhibited stronger DPPH free radical scavenging activity. The MeOH extracts of all propolis possessed stronger cytotoxicity than the corresponding water extract towards murine colon 26-L5 carcinoma and human HT-1080 fibrosarcoma cells. The result of hepatoprotective activity of Brazilian propolis on D-galactosamine (D-GalN)/tumor necrosis factor-alpha (TNF-alpha)-induced cell death in primary cultured mouse hepatocytes were found in accordance with the grade set up by beekeepers in Brazil. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.