An autonomous nutrient analyzer for oceanic long-term in situ biogeochemical monitoring.
An autonomous nutrient analyzer for oceanic long-term in situ biogeochemical monitoring.
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用于海洋长期原位生物地球化学监测的自主营养分析仪。
DOI：
10.1021/ac020696
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发表时间：
2003
影响因子：
7.4
通讯作者：
V. Garçon
中科院分区：
文献类型：
--
作者：
D. Thouron;R. Vuillemin;X. Philippon;A. Lourenço;C. Provost;A. Cruzado;V. Garçon
一种自主的原位营养分析仪(ANAIS)已经被开发出来，用于监测在海上压力(1000米以下)下部署时的硝酸盐、硅酸盐和磷酸盐的浓度。检测采用分光光度法。该仪器使用螺线管驱动的隔膜泵来推动样品、标准物质和试剂通过微管道、流动注射式恒温歧管。分析仪被放置在一个装满油的等压容器中。该分析仪工作到100bar压力，线性响应高达40微米硝酸盐、150微米硅酸盐和5微米磷酸盐，检测下限分别小于0.1微米、0.5微米和0.1微米，对硝酸盐、硅酸盐和磷酸盐的准确度分别为1、1和3%。测量方案包括13分钟内的三个步骤：用样品流冲洗、试剂导入和吸光度检测。现场测试包括仅在表层海洋中进行的硝酸盐、硅酸盐和磷酸盐测试。磷酸盐的结果还不完全令人满意。该仪器安装在YOYO垂直欧拉剖面仪上，然后成功地部署在地中海西北部，在15天时间里在200米至1100米之间采集了30个硝酸盐剖面。这种化学分析仪可以作为一种有价值的观测资产，适用于任何类型的海洋平台。
An autonomous nutrient analyzer in situ (ANAIS) has been developed to monitor nitrate, silicate, and phosphate concentrations while deployed at sea at pressure (down to 1000 m). Detection is made by spectrophotometry. The instrument uses solenoid-driven diaphragm pumps to propel the sample, the standards, and the reagents through a microconduit, flow injection-style thermostated manifold. The analyzers are placed in an equipressure container filled with oil. The analyzers operate until a pressure of 100 bar and show a linear response up to 40 microM nitrate, 150 microM silicate, and 5 microM phosphate with a detection limit less than 0.1, 0.5, and 0.1 microM and an accuracy of 1, 1, and 3% for nitrate, silicate, and phosphate, respectively. The measurement protocol includes three steps over 13 min: rinsing with the sample stream, reagents introduction, and absorbance detection. Field tests comprise ANAIS nitrate, silicate, and phosphate testing alone in the surface ocean. Phosphate results are not yet fully satisfactory. The instrument implemented on top of a YOYO vertical eulerian profiler was then deployed successfully in the northwestern Mediterranean Sea acquiring 30 nitrate profiles between 200 and 1100 m over a 15-day period. This chemical analyzer can be a valuable observing asset adapted on any type of oceanographic platform.