Nucleosome mobility and the maintenance of nucleosome positioning
Nucleosome mobility and the maintenance of nucleosome positioning
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DOI：
10.1126/science.276.5313.809
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发表时间：
1997-05-02
期刊：
影响因子：
56.9
通讯作者：
Kadonaga, JT
中科院分区：
文献类型：
--
作者：
Pazin, MJ;Bhargava, P;Kadonaga, JT
为了研究核小体的移动性和定位，将R3 lac阻遏物与三磷酸腺苷（ATP）依赖性染色质组装系统一起使用以建立五个核小体的定位，其中一个核小体位于两个R3 lac操纵子之间。当R3蛋白质与异丙基β-D-硫代半乳糖苷从DNA解离时，R3诱导的核小体位置在ATP不存在下保持不变至少60分钟，但在ATP存在下在15分钟内重排。这些结果表明，核小体是动态的和移动的，而不是静态的，DNA结合因子是持续需要的核小体定位的维护。
To study nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (ATP)-dependent chromatin assembly system to establish the positioning of five nucleosomes, with one nucleosome located between two R3 lac operators, When R3 protein was dissociated from DNA with isopropyl beta-D-thiogalactopyranoside, the R3-induced nucleosome positions remained unchanged for at least 60 minutes in the absence of ATP but rearranged within 15 minutes in the presence of ATP. These results suggest that nucleosomes are dynamic and mobile rather than static and that a DNA binding factor is continuously required for the maintenance of nucleosome positioning.