Plant regeneration from microspore-derived embryos ofBrassica Napus: Effect of embryo age, culture temperature, osmotic pressure, and abscisic acid
Plant regeneration from microspore-derived embryos ofBrassica Napus: Effect of embryo age, culture temperature, osmotic pressure, and abscisic acid
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DOI：
10.1007/bf02632058
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Bin Huang;S. Bird;R. Kemble;B. Miki;W. Keller
中科院分区：
文献类型：
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作者：
Bin Huang;S. Bird;R. Kemble;B. Miki;W. Keller
油菜的显微镜培养。Topas体外胚胎发生频率高；然而，大多数小孢子衍生的胚胎并不直接发育成植物，而通常会发生异常发育，包括在下胚轴上形成次生胚胎。目前的研究表明，年龄较大的胚胎或在较高温度（25°C）下培养的胚胎更容易发生二次胚胎发生，而在20°C下培养或在5°至10°C下预处理28天的胚胎更容易发育成正常植株。与25°C培养的胚胎相比，20°C培养的胚胎正常植株产量增加了三倍。在较低温度（5°C至10°C）下进行预处理可使正常植株的恢复率增加2至3倍。预处理过程中的高渗透力提高了低温下的胚胎存活率，但通常会抑制植物的正常发育。脱落酸无效或有害。
Microscope cultures ofBrassica napuscv. Topas undergo high frequency embryogenesis in vitro; however, the majority of microspore-derived embryos do not develop directly into plants but usually undergo abnormal development including the formation of secondary embryos on the hypocotyls. The present studies show that older embryos or embryos cultured at higher temperature (25° C) were more likely to undergo secondary embryogenesis whereas embryos cultured at 20° C or pretreated at 5° to 10° C for 28 days developed more readily into normal plants. Compared with embryos cultured at 25° C, those cultured at 20° C gave a threefold increase in normal plant production. Pretreatments at cooler temperatures (5° to 10° C) resulted in an additional two-to threefold increase in the recovery of normal plants. Higher osmoticum during pretreatment improved embryo survival at low temperatures but generally inhibited normal plant development. Abscisic acid was ineffective or deleterious.