Up-regulation of a photosystem II core protein phosphatase inhibitor and sustained D1 phosphorylation in zeaxanthin-retaining, photoinhibited needles of overwintering Douglas fir
Up-regulation of a photosystem II core protein phosphatase inhibitor and sustained D1 phosphorylation in zeaxanthin-retaining, photoinhibited needles of overwintering Douglas fir
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DOI：
10.1111/j.1365-3040.2004.01267.x
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发表时间：
2005-02-01
影响因子：
7.3
通讯作者：
Demmig-Adams, B
中科院分区：
文献类型：
--
作者：
Ebbert, V;Adams, WW;Demmig-Adams, B
常绿针叶树道格拉斯冷杉越冬针叶的叶黄素循环在耗散状态(如玉米黄质+花黄素；Z+A)的停止与光系统II(PSII)核心的D1蛋白在黎明前的强烈磷酸化状态之间存在关联。此外，PSII核心蛋白在黎明前的高度磷酸化状态与冬季比夏季PSII核心蛋白磷酸酶的亲环素样抑制物TLP40的水平显著增加有关。反过来，冬季黎明前PSII效率的下降F-v/F-m与非磷酸化形式的D1的显著下降呈正相关。与PSII核心蛋白相比，光系统II捕光复合体(LHCII)没有表现出夜间持续的磷酸化。从整个针叶中一步提取蛋白质时，花旗松夏季和冬季的总蛋白质水平是相同的。相反，越冬针叶的类囊体膜的总蛋白水平低于夏季采集的针叶，说明在越冬针叶的类囊体膜制备过程中，D1蛋白丢失。与总D1相反，在类囊体膜和全针制剂中，磷酸化和非磷酸化的D1的比例以及PSBs蛋白的水平相似。PH依赖的热量散失所需的PSBs蛋白水平在冬季有所增加，而LHCII水平保持不变。
Overwintering needles of the evergreen conifer Douglas fir exhibited an association between arrest of the xanthophyll cycle in the dissipating state (as zeaxanthin + antheraxanthin; Z + A) with a strongly elevated predawn phosphorylation state of the D1 protein of the photosystem II (PSII) core. Furthermore, the high predawn phosphorylation state of PSII core proteins was associated with strongly increased levels of TLP40, the cyclophilin-like inhibitor of PSII core protein phosphatase, in winter versus summer. In turn, decreases in predawn PSII efficiency, F-v/F-m, in winter were positively correlated with pronounced decreases in the non-phosphorylated form of D1. In contrast to PSII core proteins, the light-harvesting complex of photosystem II (LHCII) did not exhibit any nocturnally sustained phosphorylation. The total level of the D1 protein was found to be the same in summer and winter in Douglas fir when proteins were extracted in a single step from whole needles. In contrast, total D1 protein levels were lower in thylakoid preparations of overwintering needles versus needles collected in summer, indicating that D1 was lost during thylakoid preparation from overwintering Douglas fir needles. In contrast to total D1, the ratio of phosphorylated to non-phosphorylated D1 as well as the levels of the PsbS protein were similar in thylakoid versus whole needle preparations. The level of the PsbS protein, that is required for pH-dependent thermal dissipation, exhibited an increase in winter, whereas LHCII levels remained unchanged.