A millennial myosin census
A millennial myosin census
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DOI：
10.1091/mbc.12.4.780
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发表时间：
2001-04-01
影响因子：
3.3
通讯作者：
Cheney, RE
中科院分区：
文献类型：
--
作者：
Berg, JS;Powell, BC;Cheney, RE
在过去的十年里，我们对肌球蛋白超家族的大小和多样性的了解有了惊人的增长。由于这些肌动蛋白为基础的电机是候选人提供许多细胞运动的分子基础，这是必不可少的运动研究人员知道一个给定的生物体中的完整的肌球蛋白。从人类，果蝇，秀丽隐杆线虫，拟南芥，酿酒酵母，粟酒裂殖酵母，和盘基网柄霉的cDNA和/或基因组序列草案的可用性，使我们能够暂时定义和比较这些生物体中的肌球蛋白基因集。这种分析也导致了几个推定的肌球蛋白基因，可能是普遍感兴趣的识别。例如，在人类中，我们发现了总共40个已知或预测的肌球蛋白基因，包括两个新的肌球蛋白-I，三个新的II类（常规）肌球蛋白，III类/ninaC肌球蛋白的第二个成员，一个类似于XV类耳聋肌球蛋白的基因，以及一个与超家族其他成员共享至多33%同一性的新型肌球蛋白。这些肌球蛋白是除了最近发现的XVI类肌球蛋白与N-末端锚蛋白重复和两个人类基因的相似性XVIII类PDZ-肌球蛋白从小鼠。我们简要地描述了这些新认识的肌球蛋白和扩展我们以前的系统发育分析的肌球蛋白超家族，包括比较完整或几乎完整的库存肌球蛋白基因从几个实验上重要的生物体。
The past decade has seen a remarkable explosion in our knowledge of the size and diversity of the myosin superfamily. Since these actin-based motors are candidates to provide the molecular basis for many cellular movements, it is essential that motility researchers be aware of the complete set of myosins in a given organism. The availability of cDNA and/or draft genomic sequences from humans, Drosophila melanogaster, Caenorhabditis elegans, Arabidopsis thaliana, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Dictyostelium discoideum has allowed us to tentatively define and compare the sets of myosin genes in these organisms. This analysis has also led to the identification of several putative myosin genes that may be of general interest. In humans, for example, we find a total of 40 known or predicted myosin genes including two new myosins-I, three new class II (conventional) myosins, a second member of the class III/ninaC myosins, a gene similar to the class XV deafness myosin, and a novel myosin sharing at most 33% identity with other members of the superfamily. These myosins are in addition to the recently discovered class XVI myosin with N-terminal ankyrin repeats and two human genes with similarity to the class XVIII PDZ-myosin from mouse. We briefly describe these newly recognized myosins and extend our previous phylogenetic analysis of the myosin superfamily to include a comparison of the complete or nearly complete inventories of myosin genes from several experimentally important organisms.