Propionibacterium acnes and inflammation in acne:: P-acnes has T-cell mitogenic activity
Propionibacterium acnes and inflammation in acne:: P-acnes has T-cell mitogenic activity
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DOI：
10.1046/j.1365-2133.2002.04602.x
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发表时间：
2002-02-01
影响因子：
10.3
通讯作者：
Holland, KT
中科院分区：
文献类型：
--
作者：
Jappe, U;Ingham, E;Holland, KT
背景：环境证据表明痤疮丙酸杆菌在痤疮的炎症中起作用。这可能是受抗原或超抗原或促有丝分裂reactions.Objectives的目的，这项调查的目的是确定是否痤疮丙酸杆菌只有抗原活性或额外的超抗原和促有丝分裂activity.Methods淋巴细胞转化试验被用来检测反应的混合物的8个痤疮丙酸杆菌全细胞株。和它们的上清液培养液。为了确定T细胞对痤疮丙酸杆菌细胞反应的性质，在淋巴细胞转化试验中使用了小鼠抗人主要组织相容性复合物II类单克隆抗体，以抑制淋巴细胞的抗原刺激。T细胞受体（TCR）的可变区β（BV）的剧目进行了分析，使用流式细胞术的未刺激和刺激cells.Results外周血单核细胞（PBMNC）从成年人没有痤疮的历史强烈响应于静止生长期细胞的痤疮丙酸杆菌，不太强烈的细胞在指数生长期。未检测到对上清液培养液的反应。来自五个脐带血样品的PBMNC（CBMNC）在与痤疮丙酸杆菌的静止生长期细胞孵育3天和7天后反应最大。CBMNC对痤疮丙酸杆菌细胞的反应不能被封闭抗体完全抑制。对TCRBV库的分析表明，痤疮丙酸杆菌不诱导某些携带BV元件的T细胞的缺失或过度表达。在用封闭抗体预孵育后重复TCRBV分析。携带某些BV成分的T细胞发生缺失，携带某些BV成分的T细胞没有过度表达。结论提出了痤疮丙酸杆菌细胞激活淋巴细胞的两种机制，抗原驱动和促分裂原驱动。这些结果与痤疮病变中炎症的组织学证据一致。
Background Circumstantial evidence suggests that Propionibacterium acnes has a role in the inflammation of acne. This could be effected by antigenic or superantigenic or mitogenic reactions.Objectives The purpose of this investigation was to determine whether P. acnes had only antigenic activity or additional superantigenic and mitogenic activity.Methods A lymphocyte transformation assay was used to detect responses to a mixture of eight P. acnes whole cell isolates. and their supernatant culture fluids. In order to determine the nature of T-cell reactions to P. acnes cells a mouse-antihuman major histocompatibility complex class II monoclonal antibody was used in the lymphocyte transformation assay to inhibit the antigenic stimulation of lymphocytes. An analysis of the T-cell receptor (TCR) variable region beta (BV) repertoire was undertaken using flow cytometry of the unstimulated and stimulated cells.Results Peripheral blood mononuclear cells (PBMNC) from adults with no history of acne responded strongly to stationary growth phase cells of P. acnes, less strongly to cells in the exponential growth phase. No response was detected to supernatant culture fluids. PBMNC from five cord blood samples (CBMNC) responded maximally after 3 and 7 days of incubation with stationary growth phase cells of P. acnes. The reaction of CBMNC to P. acnes cells was not suppressed completely by the blocking antibody. The analysis of the TCRBV repertoire indicated that P. acnes induced no deletion or over-representation of certain BV element-bearing T cells. The TCRBV analysis was repeated after preincubation with the blocking antibody. Deletion of T cells bearing certain BV components occurred and there was no over-representation of T cells carrying certain BV components.Conclusions Two mechanisms of lymphocyte activation by P. acnes cells are proposed, antigen and mitogen driven. These results are consistent with the histological evidence of inflammation in acne lesions.