Dwarf and short grain 1, encoding a putative U-box protein regulates cell division and elongation in rice
Dwarf and short grain 1, encoding a putative U-box protein regulates cell division and elongation in rice
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DOI：
10.1016/j.jplph.2016.11.012
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发表时间：
2017-02-01
影响因子：
4.3
通讯作者：
He, Guanghua
中科院分区：
文献类型：
--
作者：
Wang, Nan;Xing, Yadi;He, Guanghua
植物激素协调植物对环境刺激的反应以及细胞分裂和伸长的内源发育程序。油菜素类固醇是植物发育中最重要的激素之一。最近，泛素-26S-蛋白酶体系统被发现在激素生物学中起着关键作用。在本研究中，我们分析了水稻基因DSG1的功能，该基因编码U-box E3泛素连接酶。DSG1突变体(Tud1的等位基因突变体)的根、节间、圆锥花序和种子的长度都比野生型短，这是由于细胞分裂和伸长缺陷所致。此外，DSG1突变体的叶片更宽、更卷曲。DSG1蛋白定位于细胞核和细胞质，并不表现出其在根、茎、叶、鞘和小穗中表达的组织特异性。DSG1突变体对油菜素类固醇的敏感性低于野生型，并且DSGI的表达受到油菜素类固醇、乙烯、生长素和水杨酸的负调控。这些结果表明，DSGI正向调节细胞分裂和伸长，可能参与多种激素途径。(C)2016年爱思唯尔股份有限公司。版权所有。
Plant hormones coordinate a plant's responses to environmental stimuli and the endogenous developmental programs for cell division and elongation. Brassinosteroids are among the most important of these hormones in plant development. Recently, the ubiquitin-26S-proteasome system was identified to play a key role in hormone biology. In this study, we analyzed the function of a rice (Oryza sativa) gene, DSG1, which encodes a U-box E3 ubiquitin ligase. In the dsg1 mutant (an allelic mutant of tud1), the lengths of the roots, internodes, panicles, and seeds were shorter than that in the wild-type, which was due to defects in cell division and elongation. In addition, the leaves of the dsg1 mutant were wider and curled. The DSG1 protein is nuclear- and cytoplasm-localized and does not show tissue specificity in terms of its expression, which occurs in roots, culms, leaves, sheaths, and spikelets. The dsg1 mutant is less sensitive to brassinosteroid treatment than the wild-type, and DSGI expression is negatively regulated by brassi-nosteroids, ethylene, auxin, and salicylic acid. These results demonstrate that DSGI positively regulates cell division and elongation and may be involved in multiple hormone pathways. (C) 2016 Elsevier GmbH. All rights reserved.