Abeta upregulates and colocalizes with LGI3 in cultured rat astrocytes.
Abeta upregulates and colocalizes with LGI3 in cultured rat astrocytes.
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发表时间：
2007
影响因子：
4
通讯作者：
N. Kimura;Y. Ishii;Shingo Suzaki;T. Negishi;S. Kyuwa;Y. Yoshikawa
中科院分区：
文献类型：
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作者：
N. Kimura;Y. Ishii;Shingo Suzaki;T. Negishi;S. Kyuwa;Y. Yoshikawa
1. 富含亮氨酸的胶质瘤失活（LGI）基因家族编码一种富含亮氨酸的重复（LRR）蛋白，这种蛋白被认为专门参与蛋白质-蛋白质和蛋白质-基质相互作用。由于淀粉样β肽（Abeta）在神经细胞中诱导另一种lrr编码基因的表达，我们评估了Abeta如何影响大鼠原代大脑皮层培养物和星形胶质细胞培养物中LGI基因的表达。RT-PCR和Western Blotting分析显示，在大鼠星形胶质细胞培养中，Abeta强烈诱导LGI3的表达。Western Blotting分析还显示，胶质纤维酸性蛋白（GFAP）和载脂蛋白E （ApoE）均与β诱导的LGI3上调同时显著升高。免疫细胞化学表明LGI3在质膜上与β共定位，也在星形胶质细胞内与β共定位。这些发现表明激活的LGI3可能参与了星形胶质细胞对β的反应。
1. The leucine-rich glioma inactivated (LGI) family of genes encodes a leucine-rich repeat (LRR) protein, proteins that are thought to be specifically involved in protein-protein and protein-matrix interactions. Since amyloid beta peptide (Abeta) has been previously shown to induce the expression of another LRR-encoding gene in neural cells, we assessed how Abeta affects LGI gene expression in rat primary cerebral cortical cultures and astrocyte cultures. Both RT-PCR and Western Blotting analyses revealed that Abeta robustly induced the expression of LGI3 in rat astrocyte cultures.2. Western Blotting analyses also showed that both glial fibrillary acidic protein (GFAP) and apolipoprotein E (ApoE) significantly increased coincidentally with the Abeta-induced upregulation of LGI3. Immunocytochemistry showed that LGI3 colocalized with Abeta at plasma membranes and also with internalized Abeta in astrocytes. These findings suggest that activated LGI3 may be involved in the astroglial response against Abeta.