Protein Corona Analysis of Silver Nanoparticles Exposed to Fish Plasma
Protein Corona Analysis of Silver Nanoparticles Exposed to Fish Plasma
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DOI：
10.1021/acs.estlett.7b00074
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发表时间：
2017-05-01
影响因子：
10.9
通讯作者：
Sepulveda, Maria S.
中科院分区：
文献类型：
--
作者：
Gao, Jiejun;Lin, Lu;Sepulveda, Maria S.
与生物流体接触的纳米颗粒（NP）经历表面化学变化，这可能影响其生物分布和下游生理影响。一种这样的变化涉及在NP表面上形成蛋白质冠（PC）。在这里，我们提出了一个基础性的研究PC的形成后，孵育的聚乙烯吡咯烷酮包被的银纳米粒子（PVP-AgNPs，50 nm）在血浆中的小口鲈鱼（Micropterus bassieu）。PC形成的水平随着暴露时间的增加而增加，并且还受性别的影响，在雄性血浆中孵育的AgNP具有比在雌性血浆中孵育的AgNP略薄的PC和比在雌性血浆中孵育的AgNP更负的电位。蛋白质组学分析还揭示了PC组成的性别特异性差异：特别是，鸡蛋特异性蛋白质（卵黄蛋白原和透明质酸）被确定为仅来自女性血浆的PC，提高其作用的可能性，银纳米颗粒相关的生殖毒性，促进其在发育中的卵母细胞的积累。
Nanoparticles (NPs) in contact with biological fluids experience changes in surface chemistry that can impact their biodistribution and downstream physiological impact. One such change involves the formation of a protein corona (PC) on the surface of NPs. Here we present a foundational study of PC formation following the incubation of polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs, 50 nm) in the plasma of smallmouth bass (Micropterus dolomieu). The level of PC formation increases with exposure time and is also affected by gender, with AgNPs incubated in male plasma having PCs slightly thinner than and potentials less negative than those of AgNPs incubated in female plasma. Proteomic analysis also revealed gender-specific differences in PC composition: in particular, egg-specific proteins (vitellogenin and zona pellucida) were identified in only PCs derived from female plasma, raising the possibility of their roles in AgNP-related reproductive toxicity by promoting their accumulation in developing oocytes.