Seasonal differences in clock gene expression levels in buccal epithelial cells of obese men: a pilot study
Seasonal differences in clock gene expression levels in buccal epithelial cells of obese men: a pilot study
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肥胖男性颊上皮细胞时钟基因表达水平的季节性差异：一项试点研究
DOI：
10.1007/s00484-021-02092-2
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发表时间：
2021
影响因子：
3.2
通讯作者：
Kanikowska Dominika
中科院分区：
文献类型：
--
作者：
Sato Maki;Hayashi Hisaki;Kanikowska Dominika
不同的生物过程显示出人类的季节性变化，包括重要的代谢途径。基因表达的季节性变化可能会影响人类免疫力的年度差异。我们假设与体重指数相关的时钟基因表达水平发生季节性变化。6名非肥胖男性和5名肥胖男性参加了夏季和冬季活动。收集两毫升唾液，从唾液中的口腔上皮细胞中提取总RNA。用实时荧光定量聚合酶链式反应检测Clock、BMAL1、PER1、CRY2、REV-ERB-α和REV-ERB-B的Clock基因表达水平。采血检测糖化血红蛋白、血糖、胰岛素、脂联素、IL-6和肿瘤坏死因子-α。参与者被问及他们的睡眠时间和季节模式。在本研究中，时钟、BMAL1和REV-ERB-β基因的表达水平在冬季显著低于夏季；BMAL1在肥胖男性的表达水平显著低于非肥胖男性。肥胖者和非肥胖者的脂联素和胰岛素水平有显著差异。HbA1c、血糖、胰岛素、脂联素、IL-6或肿瘤坏死因子-α浓度没有明显的季节性影响。夏季和冬季的睡眠时间没有显著差异。冬季的短光周期可能有助于男性时钟基因表达的季节性变化。在目前的结果中，揭示了时钟基因表达水平的季节性差异可能与肥胖有关。这些结果还显示了使用唾液样本以非侵入性方式测量时钟基因表达的可能性。
Various biological processes show seasonal variations in humans, including important metabolic pathways. Seasonal changes in gene expression may affect annual differences in human immunity. We hypothesized that seasonal changes occur in clock gene expression levels that are associated with body mass index. Six non-obese men and five obese men participated in summer and winter. Two milliliters of saliva were collected, and total RNA was isolated from buccal epithelial cells in saliva. The clock gene expression levels of CLOCK, BMAL1, PER1, CRY2, REV-ERB-α, and REV-ERB-ß were examined by real-time PCR. Blood samples were measured HbA1c, glucose, insulin, adiponectin, IL-6, and TNF-α. Participants were asked about their sleeping hours and seasonal pattern. In the present study, CLOCK, BMAL1, and REV-ERB-β gene expression levels were significantly lower in winter than in summer; BMAL1 expression level was significantly lower in obese men than in non-obese. Concentrations of adiponectin and insulin were significantly different between obese and non-obese. No significant seasonal effects were observed in HbA1c, glucose, insulin, adiponectin, IL-6, or TNF-α concentrations. Sleep duration did not significantly differ between summer and winter. The short photoperiod during winter might contribute to seasonal alterations in the expression of clock genes in men. In the present results, revealed seasonal differences in clock gene expression levels might be associated with obesity. These results also showed the potential for measuring clock gene expression in a non-invasive manner using saliva samples.