Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL/6J mice
Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL/6J mice
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DOI：
10.3109/00016489.2014.900704
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发表时间：
2014-09
影响因子：
1.4
通讯作者：
Yun Liu;Hanqi Chu;Jin Chen;Liang-qiang Zhou;Qingguo Chen;Yangping Yu;Z. Wu;Shaoli Wang;Yanbing Lai;Chunchen Pan;Yonghua Cui
中科院分区：
文献类型：
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作者：
Yun Liu;Hanqi Chu;Jin Chen;Liang-qiang Zhou;Qingguo Chen;Yangping Yu;Z. Wu;Shaoli Wang;Yanbing Lai;Chunchen Pan;Yonghua Cui
结论：C57BL/6J （C57）小鼠耳蜗外侧壁Na+- k +- 2cl -共转运体异构体1 (NKCC1) mRNA和蛋白水平随年龄增长而降低。NKCC1的下调可能影响K+转运效率和离子转运细胞的稳态，引起老年C57小鼠耳蜗细胞的不可逆损伤。我们的研究结果表明，NKCC1可能在年龄相关性听力损失（AHL）的发病机制中发挥重要作用。目的：探讨NKCC1转运蛋白的功能表达与AHL病因的关系。方法：采用C57小鼠，按年龄随机分为4组（4周、14周、26周、52周）。应用免疫荧光技术、实时荧光定量PCR和western blot检测不同年龄C57小鼠耳蜗侧壁中NKCC1的表达。结果：NKCC1在四组血管纹和螺旋韧带II型纤维细胞中均有表达。NKCC1在转录水平和蛋白水平上均随年龄的增长而降低。
Abstract Conclusions: Na+-K+-2Cl– co-transporter isoform 1 (NKCC1) mRNA and protein decrease with increasing age in the cochlear lateral wall of C57BL/6J (C57) mice. The down-regulation of NKCC1 may influence the K+ transport efficiency and the homeostasis of ion transport cells, and cause the irreversible damage of cochlear cells in old C57 mice. Our results indicate that NKCC1 may play an important role in the pathogenesis of age-related hearing loss (AHL). Objectives: The aim of the present study was to investigate the relationship between the functional expression of NKCC1 transporter and the etiology of AHL. Methods: C57 mice were used and randomly divided into four groups according to age (4 weeks, 14 weeks, 26 weeks, and 52 weeks). Immunofluorescence technique, quantitative real-time PCR, and western blot were applied to detect the expression of NKCC1 in the cochlear lateral wall of C57 mice at the various ages. Results: In all four groups, the expression of NKCC1 was observed in the stria vascularis and type II fibrocytes of the spiral ligament. Also, the expression of NKCC1 appeared to decrease with age at both the transcriptional level and the protein level.