Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds.
Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds.
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发表时间：
1999-06
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通讯作者：
F. Maquart;F. Chastang;Alain Siméon;P. Birembaut;P. Gillery;Y. Wegrowski
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作者：
F. Maquart;F. Chastang;Alain Siméon;P. Birembaut;P. Gillery;Y. Wegrowski
Centella asiatica（TECA）是一种在欧洲已使用多年的药物，用于治疗伤口愈合缺陷。它是从植物中提取的3种三萜的重组混合物，积雪草酸，羟基积雪草酸和积雪苷。在这份报告中，我们研究了TECA及其分离组分在Schilling等人描述的创伤室模型中的作用。将不锈钢创伤室手术插入大鼠皮肤下，并接受TECA或其纯化组分的连续注射。在第7、14、21或28天收集室用于生化分析或组织学检查。TECA注射的伤口室的特征在于增加的干重，DNA，总蛋白，胶原蛋白和糖醛酸含量。肽羟脯氨酸也增加，表明伤口中胶原基质的重塑增加。TECA的3种纯化组分都能够再现完整药物的效果，但因产品而异。3种三萜中活性最强的是积雪草酸和积雪苷。积雪苷产生了一个优先刺激胶原蛋白的合成，并在低剂量只有积极的。除胶原外，这3种成分也能刺激糖胺聚糖的合成。
Titrated Extract from Centella asiatica (TECA) is a drug which has been used for many years in Europe for the treatment of wound healing defects. It is a reconstituted mixture of 3 triterpenes extracted from the plant, asiatic acid, madecassic acid and asiaticoside. In this report, we studied the effects of TECA and its separated components in the wound chamber model described by Schilling et al. Stainless steel wound chambers were surgically inserted under the skin of rats and received serial injections of either TECA or its purified components. Chambers were collected at days 7, 14, 21 or 28 for biochemical analysis or histological examination. TECA-injected wound chambers were characterized by increased dry weight, DNA, total protein, collagen and uronic acid contents. Peptidic hydroproline was also increased, showing an increased remodeling of the collagen matrix in the wound. The 3 purified components of TECA were all able to reproduce the effects of the complete drug, with some differences depending on the product. Asiatic acid and asiaticoside were the most active of the 3 triterpenes. Asiaticoside exerted a preferential stimulation of collagen synthesis and was active at low doses only. In addition to collagen, the 3 components were also able to stimulate glycosaminoglycan synthesis.