Regulation of formation of volatile compounds of tea (Camellia sinensis) leaves by single light wavelength.
Regulation of formation of volatile compounds of tea (Camellia sinensis) leaves by single light wavelength.
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DOI：
10.1038/srep16858
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发表时间：
2015-11-16
影响因子：
4.6
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Fu X;Chen Y;Mei X;Katsuno T;Kobayashi E;Dong F;Watanabe N;Yang Z
光波长对植物生长发育的调控已被广泛研究。光波长对植物代谢产物形成的影响研究较少。本研究旨在探讨茶树采前与采后叶片挥发性物质的形成是否受光波长的调控。在本研究中，与自然光或黑暗处理相比，蓝光（470 nm）和红光（660 nm）显着增加了大多数内源挥发物，包括挥发性脂肪酸衍生物（VFADs），挥发性苯丙素/苯甲酰基（VPBs），和挥发性萜烯（VT）在采前茶叶。此外，蓝光和红光显著上调参与VFADs形成的9/13-脂氧合酶、参与VPBs形成的苯丙氨酸解氨酶和参与VT形成的萜烯脱氢酶的表达水平。与采前相比，单一光波长对采后叶片挥发性物质形成的影响不显著。这些结果表明，蓝光和红光可以成为重塑采前茶叶香气的有前途的技术。此外，我们的研究首次证明了光波长可以激活参与植物挥发物形成的关键基因的表达。
Regulation of plant growth and development by light wavelength has been extensively studied. Less attention has been paid to effect of light wavelength on formation of plant metabolites. The objective of this study was to investigate whether formation of volatiles in preharvest and postharvest tea (Camellia sinensis) leaves can be regulated by light wavelength. In the present study, in contrast to the natural light or dark treatment, blue light (470 nm) and red light (660 nm) significantly increased most endogenous volatiles including volatile fatty acid derivatives (VFADs), volatile phenylpropanoids/benzenoids (VPBs), and volatile terpenes (VTs) in the preharvest tea leaves. Furthermore, blue and red lights significantly up-regulated the expression levels of 9/13-lipoxygenases involved in VFADs formation, phenylalanine ammonialyase involved in VPBs formation, and terpene synthases involved in VTs formation. Single light wavelength had less remarkable influences on formation of volatiles in the postharvest leaves compared with the preharvest leaves. These results suggest that blue and red lights can be promising technology for remodeling the aroma of preharvest tea leaves. Furthermore, our study provided evidence that light wavelength can activate the expression of key genes involved in formation of plant volatiles for the first time.