Optical coherence tomography for the detection of laser in situ keratomileusis in donor corneas
Optical coherence tomography for the detection of laser in situ keratomileusis in donor corneas
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DOI：
10.1097/00003226-200301000-00011
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发表时间：
2003-01-01
期刊：
影响因子：
2.8
通讯作者：
Welge-Luessen, U
中科院分区：
文献类型：
--
作者：
Priglinger, SG;Neubauer, AS;Welge-Luessen, U
目的。 2001年，全世界进行了超过一百万例激光原位角膜磨镶术（LASIK）手术。考虑到屈光手术数量的不断增加，眼库将越来越多地面临如何识别那些接受过屈光手术的捐赠者的问题。迄今为止，尚未建立有效的筛查方法来识别供体眼中的 LASIK 手术。因此，本研究的目的是确定光学相干断层扫描（OCT）是否可用于检测 LASIK 引起的人角膜变化。方法。对 20 个器官培养的人角膜盘进行了激光原位角膜磨镶术。进行的准分子激光烧蚀范围为 0 至 12 屈光度。角膜保持培养，并在术后 6 个月内通过 OCT 评估角膜瓣-基质界面的可见度。 LASIK 手术。此外，还对两个生前有 LASIK 治疗史的供体角膜进行了结构变化筛查。结果。光学相干断层扫描能够在所有检查时间间隔检测所有角膜中角膜瓣和残留基质组织之间的界面。不同消融深度之间的信号强度没有差异。与平均基质强度相比，界面的相对信号强度范围为2.1至6.0。在疑似接受过 LASIK 手术的两个供体角膜中，OCT 扫描显示了体外模型中发现的特征性基质界面。结论。 OCT 角膜检查可能是眼库筛选先前 LASIK 手术供体角膜的合适技术。
Purpose. In 2001, more than one million laser in situ keratomileusis (LASIK) procedures were performed worldwide. Considering the increasing number of refractive procedures, eye banks will be increasingly confronted with the problem of how to identify those donors with prior refractive surgery. To date, efficient screening methods to identify LASIK surgery in dondor eyes have not been established. Therefore, the purpose of the current study was to determine whether optical coherence tomography (OCT) can be used to detect the presence of LASIK-induced changes in human corneas. Methods. Laser in situ keratomileusis was performed on 20 organ-cultured human cornea disks. The excimer laser ablation performed ranged from 0 to 12 diopters. The corneas were maintained in culture, and the visibility of flap-stromal interface by OCT was assessed up to 6 months after the. LASIK procedure. Additionally, two donor corneas with the history of LASIK treatment before death were screened for structural changes. Results. Optical coherence tomography scans were able to detect the interface between the corneal flap and the residual stromal tissue in all corneas and at all examined time intervals. There were no differences in signal intensity among the different depths of ablation. The relative signal intensity of the interface compared with the averaged stromal intensity ranged from 2.1 to 6.0. In both donor corneas with suspected prior LASIK surgery, OCT scanning showed the characteristic stromal interface as found in the in vitro model. Conclusions. Corneal examination by OCT could be an appropriate technique for eye banks to screen donor corneas for prior LASIK surgery.