Immunoselection in vitro of a non-metastatic variant from a highly metastatic tumor.
Immunoselection in vitro of a non-metastatic variant from a highly metastatic tumor.
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来自高度转移性肿瘤的非转移性变体的体外免疫选择。
DOI：
10.1002/ijc.2910270318
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发表时间：
1981
影响因子：
6.4
通讯作者：
Kerbel,RS
中科院分区：
文献类型：
--
作者：
Frost,P;Kerbel,RS
通过针对MDAY-D2的LY6.2表面标志的抗血清，实现了对DBA/2小鼠高转移的MDAY-D2肿瘤的一个非转移变种的免疫选择。虽然新的肿瘤(MDAY-D2.L61)没有表达可检测到的LY6.2，但其他可测量的表面标志物包括H2d、FCR和TAA保持不变。MDAY-D2.L61的表型行为是一种非转移性肿瘤，在体内和体外维持一年后保持稳定。现在可以使用免疫选择从不同的亲本肿瘤细胞群中获得具有稳定表型行为的变异体。
Immunoselection of a non‐metastatic variant of the highly metastatic MDAY‐D2 tumor of DBA/2 mice was achieved by means of an antiserum directed to the LY6.2 surface marker of MDAY‐D2. While the new tumor (MDAY‐D2.L61) expresses no detectable LY6.2, other measurable surface markers including H2d, FcR and TAA remain unchanged. The phenotypic behavior of MDAY‐D2.L61 is that of a non‐metastatic tumor and has remained stable after 1 year of maintenancein vivoandin vitro.Immunoselection can now be used to derive variants with stable phenotypic behavior from heterogeneous parent tumor cell populations.