Overexpression of Arabidopsis cyclin D2;1 in cotton results in leaf curling and other plant architectural modifications
Overexpression of Arabidopsis cyclin D2;1 in cotton results in leaf curling and other plant architectural modifications
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拟南芥细胞周期蛋白 D2;1 在棉花中的过度表达导致叶片卷曲和其他植物结构改变
DOI：
10.1007/s11240-012-0148-3
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发表时间：
2012-08-01
影响因子：
3
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Liu, Guanze;Jin, Shuangxia;Zhang, Xianlong
D 类细胞周期蛋白基因在控制植物发育的细胞周期中发挥着关键作用。为了评估 D 型细胞周期蛋白对植物结构的影响，通过农杆菌介导的转化，研究了由 CaMV35S 启动子驱动的拟南芥细胞周期蛋白 D2;1 (Arath;CYCD2;1) 基因在棉花中的异位表达。 Northern印迹显示cyclinD2;1基因在转基因棉花植株中高表达。表型研究表明，Arath;CYCD2;1的过度表达导致明显的叶片结构变化：与野生型相比，转基因植物的叶片表皮由更多的小细胞组成。 Arath;CYCD2;1 植物内层的叶肉细胞组织比野生型植物内层的叶肉细胞组织更松散。此外，与田间野生型植物相比，转基因植物的叶子颜色更深，叶绿素更多，光合作用速率更高。组织培养表明，在缺乏外源生长素的情况下，Arath;CYCD2;1的过表达促进了愈伤组织的形成，但抑制了细胞分化。 qRT-PCR 显示多个细胞周期相关基因，特别是 GhRBR 和 GhCYCD3;1 的转录水平受到 Arath;CYCD2;1 插入的调节。结果表明，拟南芥CyclinD2;1基因的异位表达可以调节棉花植株结构或细胞培养特性。
The D-class cyclin genes play key roles in controlling the cell cycle in plant development. To evaluate the effects of D-type cyclins on plant architecture, ectopic expression of the ArabidopsiscyclinD2;1 (Arath;CYCD2;1) gene driven by the CaMV35S promoter was investigated in cotton via Agrobacterium-mediated transformation. Northern blot showed the cyclinD2;1 gene was highly expressed in transgenic cotton plants. Phenotype investigation showed that overexpression of Arath;CYCD2;1 led to obvious leaf architecture change: the leaf epidermis of transgenic plants consist of more small cells compared to the wild-type. Mesophyll cells in the inner layers of Arath;CYCD2;1 plants were organized more loosely than those in the inner layers of wild-type plants. Moreover, transgenic plants had darker leaves, more chlorophyll, and a higher rate of photosynthesis than wild-type plants in the field. Tissue cultures indicated that the overexpression of Arath;CYCD2;1 promoted callus formation in the absence of exogenous auxin, but inhibited cell differentiation. The qRT-PCR revealed that several cell cycle–associated genes, particularly the transcript levels of GhRBR and GhCYCD3;1 were regulated by the Arath;CYCD2;1 insertion. The results implied that the cotton plant architecture or cell culture characters could be regulated by ectopic expression of the ArabidopsiscyclinD2;1 gene.