Genome and Transcriptome Analysis of Ascochyta pisi Provides Insights into the Pathogenesis of Ascochyta Blight of Pea.
Genome and Transcriptome Analysis of Ascochyta pisi Provides Insights into the Pathogenesis of Ascochyta Blight of Pea.
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豌豆壳二孢的基因组和转录组分析为豌豆壳二孢枯病的发病机制提供了见解
DOI：
10.1128/spectrum.04488-22
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发表时间：
2023-02-14
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
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子囊病是世界范围内豆科作物的主要病害。Ascochyta Pisi和其他Ascochyta种已被鉴定为Ascochyta Backation的病原菌。在这里，我们首先报道了豌豆曲霉在中国中引起的子囊病，并报道了高质量的完整注释的曲霉基因组。摘要由豌豆弯孢子囊霉(Ascochyta Pisi)引起的子囊藻疫病是对豌豆(Pisum sativum L.)全球生产。阐明豌豆黑星病菌的致病机理，将有助于选育抗病豌豆品种，为豌豆病害防治开辟有效途径。然而，目前对稻瘟病菌的基因组特征和致病因素知之甚少。在本研究中，我们首次报道了豌豆纹枯病的病原菌之一--菜豆赤霉病病原菌。用PacBio和Illumina测序技术对代表菌株A.Pisi HNA23的基因组进行了测序。HNA23基因组组件的大小几乎为41.5Mb，包含10,796个推测的蛋白质编码基因。我们预测了555个碳水化合物活性酶(CAZymes)，1008个分泌蛋白，74个富含半胱氨酸的小分泌蛋白(SSCP)和26个次生代谢物生物合成基因簇(SMGCS)。Pisi的基因组特征与其他6种囊藻基因组的特征比较表明，该属的CAZymes、分泌组和SMGCS具有相当的保守性。重要的是，进一步分析了HNA23在豌豆侵染过程中的三个阶段的转录情况。我们发现245个CAZyme和29个SSCP在所有三个测试感染阶段都上调。SMGCS也被触发，但大多数是在感染的一个阶段诱导的。综上所述，我们的研究结果为子囊藻提供了重要的基因组信息。并为进一步深入了解A.Pisi的发病机制提供了新的思路。叶枯病是世界范围内豆科作物的一种主要病害。Ascochyta Pisi和其他Ascochyta种已被鉴定为Ascochyta Backation的病原菌。在这里，我们首先报道了豌豆曲霉在中国中引起的子囊病，并报道了高质量的完整注释的曲霉基因组。通过比较基因组分析，分析了7种子囊藻的异同。我们预测在这些物种中存在丰富的CAZymes(每个物种569个)、分泌蛋白(每个物种851个)和多产的次生代谢物基因簇(每个物种29个)。我们根据植物中的转录本确定了一组可能与真菌毒力有关的基因，包括CAZymes、SSCP和次生代谢物。比较基因组分析的结果突出了子囊藻的遗传多样性，有助于理解子囊藻物种的进化关系。在植物中，转录组分析为进一步研究Ascochyta spp之间相互作用的机制提供了可靠的信息。还有豆类。
Ascochyta blight is a major disease of legumes worldwide. Ascochyta pisi and other Ascochyta species have been identified as pathogens of ascochyta blight. Here, we first report that A. pisi causes ascochyta blight of pea in China, and we report the high-quality, fully annotated genome of A. pisi. ABSTRACT Ascochyta blight caused by Ascochyta pisi is a major constraint to pea (Pisum sativum L.) production worldwide. Deciphering the pathogenic mechanism of A. pisi on peas will help in breeding resistant pea varieties and developing effective approaches for disease management. However, little is known about the genomic features and pathogenic factors of A. pisi. In this study, we first report that A. pisi is one of the causal agents of ascochyta blight disease of pea in China. The genome of the representative isolate A. pisi HNA23 was sequenced using PacBio and Illumina sequencing technologies. The HNA23 genome assembly is almost 41.5 Mb in size and harbors 10,796 putative protein-encoding genes. We predicted 555 carbohydrate-active enzymes (CAZymes), 1,008 secreted proteins, 74 small secreted cysteine-rich proteins (SSCPs), and 26 secondary metabolite biosynthetic gene clusters (SMGCs). A comparison of A. pisi genome features with the features of 6 other available genomes of Ascochyta species showed that CAZymes, the secretome, and SMGCs of this genus are considerably conserved. Importantly, the transcriptomes of HNA23 during infection of peas at three stages were further analyzed. We found that 245 CAZymes and 29 SSCPs were upregulated at all three tested infection stages. SMGCs were also trigged, but most of them were induced at only one stage of infection. Together, our results provide important genomic information on Ascochyta spp. and offer insights into the pathogenesis of A. pisi. IMPORTANCE Ascochyta blight is a major disease of legumes worldwide. Ascochyta pisi and other Ascochyta species have been identified as pathogens of ascochyta blight. Here, we first report that A. pisi causes ascochyta blight of pea in China, and we report the high-quality, fully annotated genome of A. pisi. Comparative genome analysis was performed to elucidate the differences and similarities among 7 Ascochyta species. We predict abundant CAZymes (569 per species), secreted proteins (851 per species), and prolific secondary metabolite gene clusters (29 per species) in these species. We identified a set of genes that may be responsible for fungal virulence based on transcriptomes in planta, including CAZymes, SSCPs, and secondary metabolites. The findings from the comparative genome analysis highlight the genetic diversity and help in understanding the evolutionary relationship of Ascochyta species. In planta transcriptome analysis provides reliable information for further investigation of the mechanism of the interaction between Ascochyta spp. and legumes.