Characterization of an H3N2 canine influenza virus isolated from Tibetan mastiffs in China
Characterization of an H3N2 canine influenza virus isolated from Tibetan mastiffs in China
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DOI：
10.1016/j.vetmic.2012.10.006
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发表时间：
2013-03-23
影响因子：
3.3
通讯作者：
Li, Zejun
中科院分区：
文献类型：
--
作者：
Teng, Qiaoyang;Zhang, Xu;Li, Zejun
10只3个月大的藏獒从藏獒展会上买回来两天后就发病，其中4只在两周后死亡。从死亡藏獒的肺部分离出犬流感病毒（ZJ0110），并对其进行了详细表征。序列分析表明，该犬分离株的8个基因与2007年在韩国分离的禽源犬流感病毒（H3N2）的基因最相似，两者序列一致性>98%。 ZJ0110可以通过鼻内接种和空气传播的方式实验性地感染6个月大的比格犬，导致严重的呼吸道综合症。而且，ZJ0110可以在小鼠和豚鼠的上呼吸道中复制，病毒滴度与狗上呼吸道的病毒滴度相当。尽管该病毒在基因上源自禽类，但 ZJ0110 无法通过鼻内接种实验感染鸡或鸭。这些结果表明，狗可能是禽流感病毒适应在哺乳动物中复制的中间宿主。 (C) 2012 Elsevier B.V. 保留所有权利。
Ten 3-month-old Tibetan mastiffs became ill 2 days after they were bought from a Tibetan mastiff exhibition, and 4 of them died 2 weeks later. A canine influenza virus (ZJ0110) was isolated from the lung of a deceased Tibetan mastiff and was characterized in detail. Sequence analysis indicated that the 8 genes of the canine isolate were most similar to those of avian-origin canine influenza viruses (H3N2) isolated in South Korea in 2007, with which they shared >98% sequence identity. ZJ0110 could experimentally infect 6-month-old beagles by intranasal inoculation and by airborne transmission, causing severe respiratory syndrome. Moreover, ZJ0110 could replicate in the upper respiratory tracts of mice and guinea pigs, and the virus titer was comparable to that in the upper respiratory tracts of dogs. Although the virus was genetically of avian origin, ZJ0110 could not experimentally infect chicken or ducks by intranasal inoculation. These results suggest that dogs might be an intermediary host in which avian influenza viruses adapt to replicate in mammals. (C) 2012 Elsevier B.V. All rights reserved.