Components of the spindle-assembly checkpoint are essential in Caenorhabditis elegans
Components of the spindle-assembly checkpoint are essential in Caenorhabditis elegans
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DOI：
10.1038/70309
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发表时间：
1999-12-01
影响因子：
21.3
通讯作者：
Rose, AM
中科院分区：
文献类型：
--
作者：
Kitagawa, R;Rose, AM
纺锤体组装检查点确保在有丝分裂和减数分裂期间，染色体在正确地附着到纺锤体的微管之前不会分离。在这里，我们表明，mdf-1和mdf-2的纺锤体组装检查点在秀丽隐杆线虫的组成部分，是必不可少的长期生存和生育能力的这种生物体。这些基因中的任一个的功能丧失导致各种缺陷的积累，包括染色体异常、X染色体不分离或缺失、性腺发育问题和胚胎致死。识别α 2蛋白的抗体以细胞周期依赖的方式定位于分裂胚胎的细胞核。MDF-I是一种编码与β 2 -2相互作用的产物的基因，在用诺考多唑（一种微管解聚剂）处理的减数分裂前生殖细胞中，MDF-I是细胞周期停滞和正确的染色体分离所必需的。在mdf基因产物缺失的情况下，染色体分离的错误出现并积累，最终导致遗传致死。
The spindle-assembly checkpoint ensures that, during mitosis and meiosis, chromosomes do not segregate until they are properly attached to the microtubules of the spindle. Here we show that mdf-l and mdf-2 are components of the spindle-assembly checkpoint in Caenorhabditis elegans, and are essential for the long-term survival and fertility of this organism. Loss of function of either of these genes leads to the accumulation of a variety of defects, including chromosome abnormalities, X-chromosome non-disjunction or loss, problems in gonad development, and embryonic lethality. Antibodies that recognize the MDF-2 protein localize to nuclei of the cleaving embryo in a cell-cycle-dependent manner. mdf-l, a gene encoding a product that interacts with MDF-2, is required for cell-cycle arrest and proper chromosome segregation in premeiotic germ cells treated with nocodoazole, a microtubule-depolymerizing agent. In the absence of mdf gene products, errors in chromosome segregation arise and accumulate, ultimately leading to genetic lethality.