Combination of Acellular Nerve Allograft and Human Umbilical Wharton Jell Stem Cells to Bridge Rat Femoral Nerve Defect
Combination of Acellular Nerve Allograft and Human Umbilical Wharton Jell Stem Cells to Bridge Rat Femoral Nerve Defect
复制标题
脱细胞神经同种异体移植物与人脐带沃顿胶干细胞组合修复大鼠股神经缺损
DOI：
10.1166/jbt.2016.1416
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发表时间：
2016
影响因子：
0.1
通讯作者：
Qiang Ao
中科院分区：
文献类型：
--
作者：
Aikeremujiang Muheremu;Xiyuan Wang;Zhuyi Huang;Fei Zhang;Jiang Peng;Qiang Ao
目前治疗周围神经缺损的金标准是自体神经移植。然而，在某些情况下，自体神经移植可能是不可能的，在这些情况下，脱细胞神经同种异体移植物可以作为替代品。然而，如果没有细胞支持，脱细胞神经移植物往往无法为轴突再生提供最佳条件。脱细胞神经移植物与适当的支持细胞复合后，治疗效果可能更好。为探讨人脐带间充质干细胞（human umbilical cord Wharton-derived mesenchymal stem cells，hWJMSCs）种植的去细胞神经移植物的可行性，采用hWJMSCs种植的去细胞神经移植物桥接大鼠股神经缺损，通过股四头肌重量、电生理、逆行标记和透射电镜观察，评价其治疗效果。实验结果表明，在电生理测试和电镜观察中，与不加细胞的去细胞神经移植物相比，接种hWJMSCs的去细胞神经移植物的修复效果明显更好（P0.005）。这些结果表明，种植脱细胞神经移植物与hWJMSCs可以为未来的动物和临床研究，以修复周围神经损伤的潜在方法。
Current gold standard for the treatment of peripheral nerve defect is autologous nerve grafting. However, autologous nerve grafting may not be possible in certain situations, under these circumstances, acellular nerve allografts can be used as alternatives. However, without cellular support, acellular nerve grafts often fail to provide optimal condition for axonal regeneration. Treatment effect might be much better if acellular nerve grafts were seeded with appropriate supporting cells. In the current study, to evaluate the applicability of acellular nerve grafts seeded with human umbilical cord Wharton jelly-derived mesenchymal stem cells (hWJMSCs), acellular nerve grafts seeded with hWJMSCs were used to bridge rat femoral nerve defect and the treatment effect was evaluated comparing the weight of quadriceps femoris muscle, electrophysiological tests, retrograde labeling as well as transmission electron microscopic observations. Study results showed that acellular nerve grafts seeded with hWJMSCs achieved significantly better recovery comparing to that of acellular nerve graft without any cells in electrophysiological tests and electron microscopic observations (P 005). Those results indicate that seeding acellular nerve grafts with hWJMSCs can be a potential method for future animal and clinical studies to repair peripheral nerve injury.