Targeting of P-selectin to two regulated secretory organelles in PC12 cells
Targeting of P-selectin to two regulated secretory organelles in PC12 cells
复制标题
P-选择素靶向 PC12 细胞中两个受调节的分泌细胞器
DOI：
--
复制
发表时间：
1996
影响因子：
7.8
通讯作者：
Daniel F. Cutler
中科院分区：
文献类型：
--
作者：
J. Norcott;Roberto Solari;Daniel F. Cutler
研究了P-选择素对嗜铬细胞瘤PC 12细胞调节分泌细胞器的靶向作用。通过从cDNA表达由HRP和P-选择素组成的嵌合体，然后通过亚细胞分离跟踪HRP活性，我们已经发现P-选择素包含将HRP靶向至这些细胞的突触样微泡（SLMV）以及致密核心颗粒（DCG）的信号。随后在PC 12细胞中瞬时表达的嵌合体的突变显示，在P-选择素的羧基末端胞质尾内至少有两个不同的序列是必需的，但是这两个序列都不足以运输到SLMV。其中一个序列集中在近膜C1外显子的10个氨基酸上，该外显子也涉及溶酶体靶向。运输到SLMV所需的其他序列包括蛋白质的最后四个氨基酸。相同的一系列突变对DCG靶向具有不同的影响，表明两种不同RSO的交通取决于P-选择素胞质结构域内的不同特征。
Targeting of P-selectin to the regulated secretory organelles (RSOs) of phaeochromocytoma PC12 cells has been investigated. By expressing from cDNA a chimera composed of HRP and P-selectin, and then following HRP activity through subcellular fractionation, we have discovered that P- selectin contains signals that target HRP to the synaptic-like microvesicles (SLMV) as well as the dense-core granules (DCGs) of these cells. Mutagenesis of the chimera followed by transient expression in PC12 cells shows that at least two different sequences within the carboxy-terminal cytoplasmic tail of P-selectin are necessary, but that neither is sufficient for trafficking to the SLMV. One of these sequences is centred on the 10 amino acids of the membrane-proximal C1 exon that is also implicated in lysosomal targeting. The other sequence needed for trafficking to the SLMV includes the last four amino acids of the protein. The same series of mutations have a different effect on DCG targeting, showing that traffic to the two different RSOs depends on different features within the cytoplasmic domain of P-selectin.
Rab5 对突触小泡的定位确定了突触小泡回收途径中的内体中间体。
影响因子：
6.6
作者：
FischervonMollard,G;Stahl,B;Walch-Solimena,C;Takei,K;Daniels,L;Khoklatchev,A;DeCamilli,P;Sudhof,TC;Jahn,R
通讯作者：
Jahn,R
突触小泡蛋白突触素的内吞作用需要 COOH 末端尾部。
DOI：
--
发表时间：
1991
期刊：
Journal de physiologie
影响因子：
--
作者：
Linstedt,AD;Kelly,RB
通讯作者：
Kelly,RB
DOI：
10.1073/pnas.82.12.4137
发表时间：
1985-01-01
影响因子：
11.1
作者：
JAHN, R;SCHIEBLER, W;GREENGARD, P
通讯作者：
GREENGARD, P